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Determination of biodegradation potential by two culture-independent methods in PAH-contaminated soils
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Moon, Sun Moon | - |
dc.contributor.author | Kahng, Hyung-Yeel | - |
dc.contributor.author | Kim, Jae Young | - |
dc.contributor.author | Kukor, Jerome J. | - |
dc.contributor.author | Nam, Kyoungphile | - |
dc.date.accessioned | 2009-09-07 | - |
dc.date.available | 2009-09-07 | - |
dc.date.issued | 2006-04 | - |
dc.identifier.citation | Environmental Pollution 140, 536-545 | en |
dc.identifier.issn | 0269-7491 | - |
dc.identifier.uri | https://hdl.handle.net/10371/8767 | - |
dc.description.abstract | Exiguobacterium sp. CNU020, an alkaliphile and extremohalophile bacterium, is resistant to 20 mM H2O2, a concentration that is lethal to most bacteria. Representative antioxidant genes of catalase and superoxide dismutase (SOD), obtained by PCR amplification of the genomic DNA, were characterized: the 252-bp catalase gene shared 77% similarity in the deduced amino acid sequence to that of E. oxidotolerans T-2-2(T). The 420-bp SOD gene had the closest similarity (94.3%) to the manganese-SOD of E. sibiricum 255-15. Through activity-staining analysis, stain CNU020 had at least four catalase isoforms: C1, C2, C3 and C4. Expression of each catalase isoform was dependent on the growth phase and oxidants but two catalases (C3 and C4) were always induced and expressed at a similar rate, indicating that they were constitutively expressed. RT-PCR-based expression analysis at the transcriptional level suggested that the catalase gene is strongly expressed in response to 2 mM H2O2, 0.2 mM Paraquat and 0.2 mM menadione. However, the SOD gene exhibited no observable expression pattern with 2 mM H2O2 despite its strong expression when exposed to Paraquat and menadione. | en |
dc.description.sponsorship | This research was supported by grants from the New
Jersey Hazardous Substance Management Research Center and the National Institute of Environmental Health Sciences. Additional support was provided by the KOSEF through the AEBRC at POSTECH. The authors also thank the Research Institute of Engineering Science at Seoul National University for technical assistance. | en |
dc.language.iso | en | - |
dc.publisher | Elsevier | en |
dc.subject | Catalase | en |
dc.subject | Exiguobacterium | en |
dc.subject | Extremohalophile | en |
dc.subject | Gene expression | en |
dc.subject | Superoxide diomutase | en |
dc.title | Determination of biodegradation potential by two culture-independent methods in PAH-contaminated soils | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 문희선 | - |
dc.contributor.AlternativeAuthor | 강형일 | - |
dc.contributor.AlternativeAuthor | 김재영 | - |
dc.contributor.AlternativeAuthor | 남경필 | - |
dc.identifier.doi | 10.1016/j.envpol.2005.06.028 | - |
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