S-Space College of Engineering/Engineering Practice School (공과대학/대학원) Dept. of Chemical and Biological Engineering (화학생물공학부) Journal Papers (저널논문_화학생물공학부)
Anti-inflammatory effects of silkworm hemolymph on lipopolysaccharide-stimulated macrophages
- Chang, Mi-Ra; Lee, Woong Hee; Rhee, Won Jong; Park, Tai Hyun; Kim, Eun Jeong
- Issue Date
- Springer US
- Korean Journal of Chemical Engineering Vol.30 No.9, pp. 1784-1789
- 복합학; Macrophages; Silkworm Hemolymph; Nitric Oxide (NO); Inducible Nitric Oxide Synthase (iNOS); Pro-inflammatory Cytokines
- Macrophages participate in several inflammatory pathologies such as sepsis and arthritis. We investigated the effect of silkworm hemolymph (SH) on the LPS-induced pro-inflammatory macrophages. SH inhibits LPS-induced nitric oxide (NO) production in RAW 264.7 cells and murine peritoneal macrophages. The decreased NO was reflected as a decreased amount of inducible nitric oxide synthase (iNOS) mRNA and protein. It was also found that SR inhibited pro-inflammatory cytokines, IL-1 beta, IL-6, and TNF-alpha production. To elucidate the mechanism by which SR inhibits NO production and iNOS expression, we investigated that SR suppressed I kappa B phosphorylation, which leads to the activation of NF-kappa B followed by degradation of I kappa B. This observation suggests that SH is a potential therapeutic modulator for inflammation-associated disorders.
- 0256-1115 (print)
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