Browse

Wound healing and antibacterial activities of chondroitin sulfate- and acharan sulfate-reduced silver nanoparticles

DC Field Value Language
dc.contributor.authorIm, A-Rang-
dc.contributor.authorKim, Jee Young-
dc.contributor.authorKim, Hyun-Seok-
dc.contributor.authorCho, Seonho-
dc.contributor.authorPark, Youmie-
dc.contributor.authorKim, Yeong Shik-
dc.creator김영식-
dc.date.accessioned2014-03-24T04:39:44Z-
dc.date.available2014-03-24T04:39:44Z-
dc.date.issued2013-10-
dc.identifier.citationNanotechnology Vol.24 No.39, pp. 375102-395113-
dc.identifier.issn0957-4484-
dc.identifier.urihttps://hdl.handle.net/10371/91120-
dc.descriptionOnline supplementary data available from stacks.iop.org/Nano/24/395102/mmedia-
dc.description.abstractFor topical applications in wound healing, silver nanoparticles (AgNPs) have attracted much attention as antibacterial agents. Herein, we describe a green-synthetic route for the production of biocompatible and crystalline AgNPs using two glycosaminoglycans, chondroitin sulfate (CS) and acharan sulfate (AS), as reducing agents. The synthetic approach avoids the use of toxic chemicals, and the yield of AgNPs formation is found to be 98.1% and 91.1% for the chondroitin sulfate-reduced silver nanoparticles (CS-AgNPs) and the acharan sulfate-reduced silver nanoparticles (AS-AgNPs), respectively. Nanoparticles with mostly spherical and amorphous shapes were observed, with an average diameter of 6.16 ± 2.26 nm for CS-AgNPs and 5.79 ± 3.10 nm for AS-AgNPs. Images of the CS-AgNPs obtained from atomic force microscopy revealed the self-assembled structure of CS was similar to a densely packed woven mat with AgNPs sprinkled on the CS. These nanoparticles were stable under cell culture conditions without any noticeable aggregation. An approximately 128-fold enhancement of the antibacterial activities of the AgNPs was observed against Enterobacter cloacae and Escherichia coli when compared to CS and AS alone. In addition, an in vivo animal model of wound healing activity was tested using mice that were subjected to deep incision wounds. In comparison to the controls, the ointments containing CS-AgNPs and AS-AgNPs stimulated wound closure under histological examination and accelerated the deposition of granulation tissue and collagen in the wound area. The wound healing activity of the ointments containing CS-AgNPs and AS-AgNPs are comparable to that of a commercial formulation of silver sulfadiazine even though the newly prepared ointments contain a lower silver concentration. Therefore, the newly prepared AgNPs demonstrate potential for use as an attractive biocompatible nanocomposite for topical applications in the treatment of wounds.-
dc.description.sponsorshipThis work was supported by a MRC grant funded by the Korean government (MEST) (No. 2009-93146) and the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2012R1A1A2042224).-
dc.language.isoenen
dc.publisherIOP Publishingen
dc.subject의약학en
dc.titleWound healing and antibacterial activities of chondroitin sulfate- and acharan sulfate-reduced silver nanoparticlesen
dc.typeArticle-
dc.author.alternative임아랑-
dc.author.alternative김지영-
dc.author.alternative김현석-
dc.author.alternative조선호-
dc.author.alternative박유미-
dc.author.alternative김영식-
dc.identifier.doi10.1088/0957-4484/24/39/395102-
dc.citation.journaltitleNanotechnology-
dc.description.srndOAIID:oai:osos.snu.ac.kr:snu2013-01/102/0000001731/13-
dc.description.srndSEQ:13-
dc.description.srndPERF_CD:SNU2013-01-
dc.description.srndEVAL_ITEM_CD:102-
dc.description.srndUSER_ID:0000001731-
dc.description.srndADJUST_YN:N-
dc.description.srndEMP_ID:A000864-
dc.description.srndDEPT_CD:371-
dc.description.srndCITE_RATE:3.842-
dc.description.srndFILENAME:nanotechnology-wound healing (ypark).pdf-
dc.description.srndDEPT_NM:제약학과-
dc.description.srndEMAIL:kims@snu.ac.kr-
dc.description.srndSCOPUS_YN:Y-
dc.description.srndCONFIRM:Y-
dc.identifier.rimsid1538-
dc.identifier.srnd2013-01/102/0000001731/13-
dc.type.rimsART-
Appears in Collections:
College of Pharmacy (약학대학)Dept. of Manufacturing Pharmacy (제약학과)Journal Papers (저널논문_제약학과)
Files in This Item:
There are no files associated with this item.
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse