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Characterization and complete genome sequence of the Shigella bacteriophage pSf-1

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dc.contributor.authorJun, Jin Woo-
dc.contributor.authorKim, Ji Hyung-
dc.contributor.authorShin, Sang Phil-
dc.contributor.authorHan, Jee Eun-
dc.contributor.authorChai, Ji Young-
dc.contributor.authorPark, Se Chang-
dc.creator박세창-
dc.date.accessioned2014-04-01T02:16:55Z-
dc.date.available2014-04-01T02:16:55Z-
dc.date.issued2013-12-
dc.identifier.citationResearch in Microbiology Vol.164 No.10, pp. 979-986-
dc.identifier.issn0923-2508-
dc.identifier.urihttps://hdl.handle.net/10371/91275-
dc.description.abstractShigellosis is a global health problem, and Shigella flexneri is the major cause of this disease. In this study, we isolated a virulent Siphoviridae bacteriophage (phage), pSf-1, that infects S. flexneri. This phage was isolated from the Han River in Korea and was found to infect S. flexneri, Shigella boydii, and Shigella sonnei. One-step growth analysis revealed that this phage has a short latent period (10 mm) and a large burst size (86.86 PFU/cell), indicating that pSf-1 has good host infectivity and effective lytic activity. The double-stranded DNA genome of pSf-1 is composed of 51,821 bp with a G C content of 44.02%. The genome encodes 94 putative ORFs, 71 putative promoters, and 60 transcriptional terminator regions. Genome sequence analysis of pSf-1 and comparative analysis with the homologous Shigella phage Shfl1 revealed that there is a high degree of similarity between pSf-1 and Shfl1 in 54 of the 94 ORFs of pSf-1. The results of this investigation indicate that pSf-1 is a novel Shigella phage and that this phage might have potential uses against shigellosis.en
dc.description.sponsorshipThis study was financially supported by Basic Science Research Program (2010-0016748) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology, and by Korea Research Foundation Grant (KRF-2008-331-E00385).-
dc.language.isoenen
dc.publisherElsevier Masson SASen
dc.subject농수해양en
dc.subjectShigellosis-
dc.subjectShigella flexneri-
dc.subjectSiphoviridae-
dc.subjectBacteriophage-
dc.subjectpSf-1-
dc.titleCharacterization and complete genome sequence of the Shigella bacteriophage pSf-1en
dc.typeArticle-
dc.author.alternative전진우-
dc.author.alternative김지형-
dc.author.alternative신상필-
dc.author.alternative한지은-
dc.author.alternative채지영-
dc.author.alternative박세창-
dc.identifier.doi10.1016/j.resmic.2013.08.007-
dc.citation.journaltitleResearch in Microbiology-
dc.description.srndOAIID:oai:osos.snu.ac.kr:snu2013-01/102/0000030777/13-
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dc.description.srndPERF_CD:SNU2013-01-
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dc.description.srndUSER_ID:0000030777-
dc.description.srndADJUST_YN:Y-
dc.description.srndEMP_ID:A076079-
dc.description.srndDEPT_CD:551-
dc.description.srndCITE_RATE:2.889-
dc.description.srndFILENAME:1-s2.0-s0923250813001599-main.pdf-
dc.description.srndDEPT_NM:수의학과-
dc.description.srndEMAIL:parksec@snu.ac.kr-
dc.description.srndSCOPUS_YN:Y-
dc.description.srndCONFIRM:Y-
dc.identifier.srnd2013-01/102/0000030777/13-
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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