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Characterization and complete genome sequence of the Shigella bacteriophage pSf-1
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jun, Jin Woo | - |
dc.contributor.author | Kim, Ji Hyung | - |
dc.contributor.author | Shin, Sang Phil | - |
dc.contributor.author | Han, Jee Eun | - |
dc.contributor.author | Chai, Ji Young | - |
dc.contributor.author | Park, Se Chang | - |
dc.creator | 박세창 | - |
dc.date.accessioned | 2014-04-01T02:16:55Z | - |
dc.date.available | 2014-04-01T02:16:55Z | - |
dc.date.issued | 2013-12 | - |
dc.identifier.citation | Research in Microbiology Vol.164 No.10, pp. 979-986 | - |
dc.identifier.issn | 0923-2508 | - |
dc.identifier.uri | https://hdl.handle.net/10371/91275 | - |
dc.description.abstract | Shigellosis is a global health problem, and Shigella flexneri is the major cause of this disease. In this study, we isolated a virulent Siphoviridae bacteriophage (phage), pSf-1, that infects S. flexneri. This phage was isolated from the Han River in Korea and was found to infect S. flexneri, Shigella boydii, and Shigella sonnei. One-step growth analysis revealed that this phage has a short latent period (10 mm) and a large burst size (86.86 PFU/cell), indicating that pSf-1 has good host infectivity and effective lytic activity. The double-stranded DNA genome of pSf-1 is composed of 51,821 bp with a G C content of 44.02%. The genome encodes 94 putative ORFs, 71 putative promoters, and 60 transcriptional terminator regions. Genome sequence analysis of pSf-1 and comparative analysis with the homologous Shigella phage Shfl1 revealed that there is a high degree of similarity between pSf-1 and Shfl1 in 54 of the 94 ORFs of pSf-1. The results of this investigation indicate that pSf-1 is a novel Shigella phage and that this phage might have potential uses against shigellosis. | en |
dc.description.sponsorship | This study was financially supported by Basic Science Research Program (2010-0016748) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology, and by Korea Research Foundation Grant (KRF-2008-331-E00385). | - |
dc.language.iso | en | en |
dc.publisher | Elsevier Masson SAS | en |
dc.subject | 농수해양 | en |
dc.subject | Shigellosis | - |
dc.subject | Shigella flexneri | - |
dc.subject | Siphoviridae | - |
dc.subject | Bacteriophage | - |
dc.subject | pSf-1 | - |
dc.title | Characterization and complete genome sequence of the Shigella bacteriophage pSf-1 | en |
dc.type | Article | - |
dc.author.alternative | 전진우 | - |
dc.author.alternative | 김지형 | - |
dc.author.alternative | 신상필 | - |
dc.author.alternative | 한지은 | - |
dc.author.alternative | 채지영 | - |
dc.author.alternative | 박세창 | - |
dc.identifier.doi | 10.1016/j.resmic.2013.08.007 | - |
dc.citation.journaltitle | Research in Microbiology | - |
dc.description.srnd | OAIID:oai:osos.snu.ac.kr:snu2013-01/102/0000030777/13 | - |
dc.description.srnd | SEQ:13 | - |
dc.description.srnd | PERF_CD:SNU2013-01 | - |
dc.description.srnd | EVAL_ITEM_CD:102 | - |
dc.description.srnd | USER_ID:0000030777 | - |
dc.description.srnd | ADJUST_YN:Y | - |
dc.description.srnd | EMP_ID:A076079 | - |
dc.description.srnd | DEPT_CD:551 | - |
dc.description.srnd | CITE_RATE:2.889 | - |
dc.description.srnd | FILENAME:1-s2.0-s0923250813001599-main.pdf | - |
dc.description.srnd | DEPT_NM:수의학과 | - |
dc.description.srnd | EMAIL:parksec@snu.ac.kr | - |
dc.description.srnd | SCOPUS_YN:Y | - |
dc.description.srnd | CONFIRM:Y | - |
dc.identifier.srnd | 2013-01/102/0000030777/13 | - |
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