인삼이 CCl4에 의한 백서간손상 및 방사선 장해에 미치는 영향에 대하여

Abstract

Following experiments were conducted for the study of Panax Ginseng effect on Cel induced liver injury and on X•irradiated damage of albino rats. Male rats of Wistar hybrid, weighing 150±10g, were divided into 6 groups and first 3 groups were control, CCI. gh'en, :md the previous Ginseng decoction given and CCl. administered animals, and another 3 groups were control, X-irradiated, and the previous Ginseng decoction administered and X-irradiated ones. Animals were sacrificed 18 hours after CCl. administration or 24 hours after X-irradiation with the dose of 800R. Liver s were removed immediately for the determination of contents of ATP, nucleic acids and lipids as well as for the incorporation of acetate-1-HC into fatty acids in liver slices. Following results were obtained. 1) The ATP content of CCl. poisoned liver was 12.4±1.4r/ g, show ing much less than 32. I±3. 5r/ g of control value, and that of Ginseng and CCl. administered group was 20.6±8r/g, it was thus shown that the previous administration of Ginseng lessened the decrease in hepatic ATP content of CCI, given animals. 2) No appreciable alteration was observed in the hepatic nucleic acid content by the CCI, treatment with the consequence of no Ginseng effect. 3) The mitochondrial RNA content of CCμ poisoned liver was not altered 18 hours after CCI. administration. 4) The triglyceride content of CCl. poisoned rats was 15. 2±4. 2mg/g which was lowered to 9. 8±2. 9 mg/g by the previous Ginseng administration. The hepatic cholesterol content of CCI. given rats was 10.3±4. Omg/g, comparing with 6. 4±1. Omg/g of normal value, and the phospholipid content (32.3± 6.1mg/g) of CCI. poisoned liver was decreased as compared to 38. 9±4. 8mg/g of control value. The previous administration of Ginseng caused to normalize significantly such a change of cholesterol and phospholipid content. 5) The incorporation of acetate-I-HC into the triglyceride was much increased with cpm of 1.821 ±325 in liver slices of CCI, poisoned rats, as compared to the control value of 1.072±247. The synthesis of fatty acids was thus shown to be increased , reflecting the marked increase in hepatic triglyceride in CCl. administered rats. This isotopic incorporation rate in liver slices of Ginseng administered group was shown to be intermediate between the value인 of normal and Cel, treated animals.