거세가 흰쥐 뇌하수체 LH 세포에 미치는 면역조직화학적 연구

Abstract

Pituitary glands of male rats, castrated for varying lengths of time, between 7 and 150 days after operation, have been studied for their histological changes using the peroxidase-labeled antibody method. Number of LH cells reacted specifically with antiHCG and anti-RGG-peroxidase were counted from one hundred visual fields of twenty representative level of sections under higher magnification. There was a tendency that the LH cells increase gradually after castration. At the thirty days after castration, castration cells with vacuole begin to appear and reached up to 30% of total cell count at the 150 days after castration. On the other hand the pituitary glands of castrated rats which were administered testosterone propionate and testosterone cyclopentylpropionate showed marked decrease of LH cells not only in number but in the intensity of staining. Proliferation of additional LH cells were found mainly in the central zone of pituitary gland , while the LH cells in the peripheral zone remained continuously. The weight of pituitary glands of castrated rats revealed a tendency of gradual increase while that of the hormone treated castrated rats showed a tendency of gradual decrease. Those cellular changes are illustrated and their possible significance is discussed.