토끼 소화관벽 임파조직의 용혈소 형성반응에 관한 면역학적 연구

Abstract

As an experiment for the study of bursa equivaleot organ in mammalia, the present experiment was performed on the GALT of young adult rabbits whether the cells of it respond to the antigenic stimulation or not. The experimental animals, weighed 1. 5 to 1.8kg, were randomly divided into two groups. Experimental group received a single injection of 1 ml of 20% sheep erythrocytes intravenously. Control group received a single injection of 1 ml of saline solut ion intravenously. After the injection of the antigen (or saline) the animals at various interval (from 1st day to 21st day) and the palatine tonsil, Peyer's patch, appendix, spleen , and thymus were removed. The cell suspensions obtained from each organ was used for the plaque technique and the paraffin sections obtained from each organ were carried out for the staining with methyl-green pyronin, silver, and hematoxylin-eosin. Hemolysin-producing cells were determined using a modification of Jerne plaque technique and the results were expressed as plaque-forming cells per 10' cells. The results were as followings: 1. In the experimental group the spleen and palatine tonsil produced a number of plaqueforming cells. It reached to the maximum at 3 days after the injection of the antigen and then decreased gradually. 2. By contrast, thymus, appendix, and PeyerS patch did not produce the plaque•forming cells as did the spleen and palatine tonsil. 3. The number of large pyroninophilic cells were markedly increased in the extrafollicular area of palatine tonsil and the periarteriallymphocyte sheath of spleen after the antigenic stimulation. It reached to the maximum at 3 days after the injection of antigen and then decreased gradually. 4. Number of large pyroninophilic cells did not change in the appendix, Peyers patch, and thymus after the antigenic stimulation. 5. The diffuse lymphatic tissue of the extrafolli cular area of palatine tonsil were more well developed than that of appendix and PeyerS patch. These results suggest that the palatine tonsil in the rabbits as well as spleen responds to the antigenic stirn ulation, while the appendix and Peyers patch do not.