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Antibody-Based Surface Plasmon Resonance Detection of Intact Viral Pathogen

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dc.contributor.authorBaac, Hyoungwon-
dc.contributor.authorHajos, Jozsef P.-
dc.contributor.authorLee, Jennifer-
dc.contributor.authorKim, Donghyun-
dc.contributor.authorKim, Sung June-
dc.contributor.authorShuler, Michael L.-
dc.date.accessioned2009-09-16-
dc.date.available2009-09-16-
dc.date.issued2006-07-
dc.identifier.citationBiotechnol Bioeng 94:815-819en
dc.identifier.issn0006-3592-
dc.identifier.urihttps://hdl.handle.net/10371/9377-
dc.description.abstractSurface plasmon resonance (SPR) technique
was used to directly detect an intact form of insect
pathogen: the baculovirus, Autographa californica multiple
nuclear polyhedrosis virus (AcMNPV). An SPR sensor
chip with three bio-functional layerswasusedto detect the
intact AcMNPV: amine-reactive crosslinker with a disulfide
bond that chemisorbs to gold film, Protein A, and a
mouse IgG monoclonal antibody raised against a surface
protein of the target viral pathogen. A two-channel
(reference & test) micro-fluidic SPR system is used
for reliable measurement. Bio-specific response to the
AcMNPV is compared with the response for tobacco
mosaic virus (TMV) as control. Successive exposure of the
sensor chip to both viruses verifies a specific response to
AcMNPV. This serves as a prerequisite to the development
of a new type of viral pathogen detection sensors.
en
dc.description.sponsorshipThe authors are grateful to Dr. Mandy Esch (Cornell Nanoscale
Facility, Cornell University) for valuable advice on fabrication and
surface chemistry, Dr. Gary Blissard (Boyce Thompson Institute,
Cornell University) for kindly providing AcMNPV baculovirus and
AcV1 monoclonal antibody producing hybridoma cell line, and Dr.
Milton Zaitlin (Department of Plant Pathology, Cornell University) for kindly providing tobacco mosaic virus. This work was partly supported
by DARPA through CBOM, and also partly by joint research program
of Cornell Biomedical Engineering Program and Nano Bioelectronics
and Systems Research Center of Seoul National University through
KOSEF. M.L.S. acknowledges support as a NYSTAR Distinguished
Professor through the New York State Office of Science, Technology
and Academic Research.
en
dc.language.isoenen
dc.publisherJohn Wiley & Sonsen
dc.subjectintact pathogenen
dc.subjectsurface plasmon resonanceen
dc.subjectbaculovirusen
dc.subjecttobacco mosaic virusen
dc.titleAntibody-Based Surface Plasmon Resonance Detection of Intact Viral Pathogenen
dc.typeArticleen
dc.contributor.AlternativeAuthor박형원-
dc.contributor.AlternativeAuthor김동현-
dc.contributor.AlternativeAuthor김성준-
dc.identifier.doi10.1002/bit.20882-
Appears in Collections:
College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Electrical and Computer Engineering (전기·정보공학부)Journal Papers (저널논문_전기·정보공학부)
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