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Antibody-Based Surface Plasmon Resonance Detection of Intact Viral Pathogen
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Baac, Hyoungwon | - |
dc.contributor.author | Hajos, Jozsef P. | - |
dc.contributor.author | Lee, Jennifer | - |
dc.contributor.author | Kim, Donghyun | - |
dc.contributor.author | Kim, Sung June | - |
dc.contributor.author | Shuler, Michael L. | - |
dc.date.accessioned | 2009-09-16 | - |
dc.date.available | 2009-09-16 | - |
dc.date.issued | 2006-07 | - |
dc.identifier.citation | Biotechnol Bioeng 94:815-819 | en |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.uri | https://hdl.handle.net/10371/9377 | - |
dc.description.abstract | Surface plasmon resonance (SPR) technique
was used to directly detect an intact form of insect pathogen: the baculovirus, Autographa californica multiple nuclear polyhedrosis virus (AcMNPV). An SPR sensor chip with three bio-functional layerswasusedto detect the intact AcMNPV: amine-reactive crosslinker with a disulfide bond that chemisorbs to gold film, Protein A, and a mouse IgG monoclonal antibody raised against a surface protein of the target viral pathogen. A two-channel (reference & test) micro-fluidic SPR system is used for reliable measurement. Bio-specific response to the AcMNPV is compared with the response for tobacco mosaic virus (TMV) as control. Successive exposure of the sensor chip to both viruses verifies a specific response to AcMNPV. This serves as a prerequisite to the development of a new type of viral pathogen detection sensors. | en |
dc.description.sponsorship | The authors are grateful to Dr. Mandy Esch (Cornell Nanoscale
Facility, Cornell University) for valuable advice on fabrication and surface chemistry, Dr. Gary Blissard (Boyce Thompson Institute, Cornell University) for kindly providing AcMNPV baculovirus and AcV1 monoclonal antibody producing hybridoma cell line, and Dr. Milton Zaitlin (Department of Plant Pathology, Cornell University) for kindly providing tobacco mosaic virus. This work was partly supported by DARPA through CBOM, and also partly by joint research program of Cornell Biomedical Engineering Program and Nano Bioelectronics and Systems Research Center of Seoul National University through KOSEF. M.L.S. acknowledges support as a NYSTAR Distinguished Professor through the New York State Office of Science, Technology and Academic Research. | en |
dc.language.iso | en | en |
dc.publisher | John Wiley & Sons | en |
dc.subject | intact pathogen | en |
dc.subject | surface plasmon resonance | en |
dc.subject | baculovirus | en |
dc.subject | tobacco mosaic virus | en |
dc.title | Antibody-Based Surface Plasmon Resonance Detection of Intact Viral Pathogen | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 박형원 | - |
dc.contributor.AlternativeAuthor | 김동현 | - |
dc.contributor.AlternativeAuthor | 김성준 | - |
dc.identifier.doi | 10.1002/bit.20882 | - |
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