동종갑상선추출물과 Adjuvant의 반복주사로 인한 가토의 혈액상 및 임파구모세포화에 관한 연구

Abstract

Recent interests h. autoimmune diseases with investigations on its ifIh…mological character elucidated the nature of variou.; human disorders such as Sj6rgens disease, S. L. c. pernicious anemia. rheum atoid arthritis, chronic thyroiditis. especially of Hashirr.oto s lyphocytic ty /,c and other diseases, yet the pathogenesis is not fu1Jy clarified The authors attempted an experimental study for further elucidation of the pathogenesis of autoimmune thyroiditis. The lymphocytes from rabbits sensitized with homologous thyroid extract and/or adjuvant was cultured in the presence of homologous thyroid and the peripheral blood pictures during the sensiti zation antigen and non-specific stimulant PHA. The rates of lymphocyte transformation in 72• hour cultures were analyzed. The results are summerized as follows: I. Changes of hemoglobin values and peripheral leucocytosis of each experimental group were slight, however the leucocytosis and relative lymphocytopenia were observed in the group sensitized with a mixture of homologous thyroid extract and Freunds adjuvant 2· The transformation of peripheral blood lymphocytes of normal rabbits in the presence of homol ogous thyroid extract was 、ery few and almost negl igible 3. Lymphocytes of rabbits sensitized with thyroid ext;:-act showed evidently higher transformation rate than that of unsensitize:l normal rabbits in the present:e of homologous thyroid extract, he'; e'.'er these lymphocytes showed almost same degree of transformation rates in the presence of PHA as in unsensitized normal groups of rabbits. 4. Transformation rates of lymphocytes of either group sensitized with Freunds adjuvant or homologous thyroid extract showed similar results when cultureJ with either the thyroid extract or PHA in vitro 5. Lymphocytes from the rabbits sensitized with a mixture of homologous thyroid extract and Freund S adjuvant gave higher transformation rates than that of any other groups. 6. Lymphocytes sensitized with homologous thyroid extract showed variable rates of transformation according to the indivisuality of the rabbits. It is thought that these variabilities are due probably to the degree of sensitization or susceptibility of indi visual rabbit to the PHA or the antigens. It is summerized that the rate of blastogenesis of rabbit lymphocytes stimulated by thyroid extract and cultured with thyroid extract or PHA is similar to that of adjuvant stimulated, while that of thyroid extract with adjuvant is remarkably higher than 'the above two experimental groups. This results suggest that the specific and non-specific stimulation of T -lymphocytes may be closely related to the pathogenesis of autoimmune thyroiditis.