Browse

A recombinant Eimeria protein inducing interferon-γ production: comparison of different gene expression systems and immunization strategies for vaccination against coccidiosis

DC Field Value Language
dc.contributor.authorLillehoj, Hyun S.-
dc.contributor.authorChoi, Kang D.-
dc.contributor.authorJenkins, Mark C.-
dc.contributor.authorVakharia, Vikram N.-
dc.contributor.authorSong, Ki D.-
dc.contributor.authorHan, Jae Yong-
dc.contributor.authorLillehoj, Erik P.-
dc.date.accessioned2017-01-10T01:35:25Z-
dc.date.available2017-01-10T01:35:25Z-
dc.date.issued2000-
dc.identifier.citationAvian Diseases, vol.44 no.2, pp. 379-389ko_KR
dc.identifier.issn0005-2086-
dc.identifier.urihttps://hdl.handle.net/10371/99035-
dc.description.abstractSUMMARY. A rabbit antiserum against an 18- to 27-kD native protein fraction (F3)
from Eimeria acervulina merozoites identified a cDNA (3-1E) containing a 1086-base pair
insertion with an open reading frame of 170 amino acids (predicted molecular weight,
18,523). The recombinant3 -1E cDNA expressedi n Escherichiaco li produceda 60-kD fusion
protein and a 23-kD protein after factor Xa treatment of the fusion protein. Both proteins
were reactive with the F3 antiserum by western blot analysis. A rabbit antiserum against a
synthetic peptide deduced from the amino acid sequence of the 3-1E cDNA reacted with a
27-kD recombinant 3-1E protein expressed in Sf9 insect cells and a 20-kD native protein
expressed by E. acervulina sporozoites and Eimeria tenella sporozoites and merozoites. By
immunofluorescence staining, a monoclonal antibody produced against the recombinant 3-
1E protein reacted with sporozoites and merozoites of E. acervulina, E. tenella, and Eimeria
maxima. Spleen lymphocytes from E. acervulina-immune chickens showed antigen-specific
proliferation and interferon (IFN)-y production upon stimulation with the recombinant 3-
1E protein, indicating that the protein activates cell-mediated immunity during coccidiosis.
Immunizationo f chickensw ith either the E. coli- or Sf9-expressedr ecombinant3 -1E protein
with adjuvant, or direct injection of the 3-1E cDNA, induced protective immunity against
live E. acervulina. Simultaneous injection of the recombinant 3-1E protein, or the 3-1E
cDNA, with cDNAs encoding chicken IFN-y or interleukin (IL)-2/15 further enhanced
protective immunity. These results indicate that the recombinant E. acervulina 3-1E cDNA
or its polypeptide product may prove useful as vaccines against avian coccidiosis.
ko_KR
dc.language.isoenko_KR
dc.publisherAmerican Association of Avian Pathologistsko_KR
dc.titleA recombinant Eimeria protein inducing interferon-γ production: comparison of different gene expression systems and immunization strategies for vaccination against coccidiosisko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor한재용-
dc.identifier.doi10.2307/1592553-
Appears in Collections:
College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
Files in This Item:
There are no files associated with this item.
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse