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Pre-treatment of donor with 1-deamino-8-d-arginine vasopressin could alleviate early failure of porcine xenograft in a cobra venom factor treated canine recipient

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dc.contributor.authorKang, Hee Jung-
dc.contributor.authorLee, Gene-
dc.contributor.authorKim, Ji Yeon-
dc.contributor.authorLee, Seung Hee-
dc.contributor.authorWi, Hyun Cho-
dc.contributor.authorHwang, Pil Gyu-
dc.contributor.authorChung, Doo Hyun-
dc.contributor.authorKim, Young Tae-
dc.date.accessioned2009-09-28T10:00:51Z-
dc.date.available2009-09-28T10:00:51Z-
dc.date.issued2005-06-29-
dc.identifier.citationEur J Cardiothorac Surg 2005;28:149-56en
dc.identifier.issn1010-7940-
dc.identifier.urihttps://hdl.handle.net/10371/9945-
dc.description.abstractOBJECTIVE: Unlike cardiac or renal xenotransplants, the depletion of complement using cobra venom factor (CVF) does not improve pulmonary xenograft survival. Several cases suggest that the swine von Willebrand factor (vWF) may play a major role in presenting a different pathogenesis of pulmonary xenograft dysfunction from other organs. To evaluate the role of vWF and the complement system in mediating hyperacute vascular injury of pulmonary xenografts and elucidate pathogenesis of the injury, we performed swine-to-canine orthotropic single lung xenotransplantation after pre-treatment of 1-deamino-8-d-arginine vasopressin (DDAVP) and CVF. METHODS: We set up three groups for lung xenotransplantation: group I served as the control group; group II, recipients pre-treated with CVF; group III, donors pre-treated with DDAVP (9 mg/kg, 3 days)/recipients pre-treated with CVF (60 u/kg). Hemodynamic data, coagulation and complement system parameters, and grafted lung pathologies were examined serially for 3h after transplantation. RESULTS: DDAVP infusion reduced the vWF content in swine lung tissue in vivo (7.7+/-2.4 AU/mg vs 16.0+/-5.6 AU/mg, P < 0.0001). Infusion of CVF 24 h prior to transplantation effectively depleted the recipient's serum C3 and complement hemolytic activity below the detectable range. Regardless of the use of CVF, both groups I and II transplanted with unmodified grafts showed an immediate drop in leukocytes and platelet counts after transplantation. However, in group III, in recipients transplanted with DDAVP pre-treated swine lung, the platelet count did not decrease after transplantation (P = 0.0295). The decrease of plasma antithrombin and fibrinogen tended to be attenuated in group III. Light microscopic examination revealed extensive vascular thromboses in both capillary and larger vessels, as well as early pulmonary parenchymal damage in groups I and II, but were rarely observed in group III. CONCLUSIONS: Complement inhibition alone was not enough to alleviate intravascular thrombosis, the main pathology in pulmonary xenotransplantation. Pre-infusion of DDAVP to the donor animal was effective in preventing platelet sequestration and attenuated intravascular thrombosis. It is suggested that the strategies targeting vWF would be promising for successful pulmonary xenotransplantation.en
dc.description.sponsorshipThis study was supported by a grant of the Korea Health
21 R&D Project, Ministry of Health and Welfare, Republic of
Korea (Project No.: 0405-BO02-0205-0001) and National R&D
Program Grant of The Ministry of Science and Technology
(M1031006007-03B4606-00710)
en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectHyperacute rejectionen
dc.subjectPulmonary xenotransplantationen
dc.subjectPlateleten
dc.subjectDesmopressinen
dc.subjectThrombosisen
dc.titlePre-treatment of donor with 1-deamino-8-d-arginine vasopressin could alleviate early failure of porcine xenograft in a cobra venom factor treated canine recipienten
dc.typeArticleen
dc.contributor.AlternativeAuthor강희정-
dc.contributor.AlternativeAuthor이진-
dc.contributor.AlternativeAuthor김지연-
dc.contributor.AlternativeAuthor이승희-
dc.contributor.AlternativeAuthor위현조-
dc.contributor.AlternativeAuthor황필규-
dc.contributor.AlternativeAuthor정두현-
dc.contributor.AlternativeAuthor김영태-
dc.identifier.doi10.1016/j.ejcts.2005.02.042-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Pathology (병리학전공)Journal Papers (저널논문_병리학전공)
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