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Production of quail (Coturnix japonica) germline chimeras by transfer of gonadal primordial germ cells into recipient embryos

Cited 35 time in Web of Science Cited 34 time in Scopus
Authors
Kim, Mi A.; Park, Tae Sub; Kim, Jin Nam; Park, Hyun Jeong; Lee, Young Mok; Ono, Tamao; Lim, Jeong Mook; Han, Jae Yong
Issue Date
2005
Publisher
Elsevier
Citation
Theriogenology, vol.63 no.3, pp. 774-782
Keywords
QuailPrimordial germ cellGermline chimeraGermline transmission
Abstract
The possibility of producing quail germline chimeras by the transfer of gonadal primordial germ cells (gPGCs) into recipient embryos was investigated. Japanese quail of the black (D: homozygous for the autosomal incomplete dominant gene D) and wild-type plumage (WP: dþ/dþ) strains were used as donors and recipients, respectively. Gonadal cells were retrieved from the gonads of 5-dayold D embryos, and gPGCs were enriched by magnetism-activated cell sorting. Fresh (noncultured) gPGCs or those isolated after culture for 3 days with gonadal stromal cells present in the mixed cell population were introduced into the dorsal aorta of 2-day-old recipient WP embryos. Hatchability of the recipient embryos was 23.7% (31/131) and 34.4% (31/90) for those transfused with cultured or noncultured gPGCs, respectively. Of the hatched quail, 28 acquired sexual maturity; among these animals, 7.1% (1/14) and 21.4% (3/14) of those that received cultured or noncultured gPGCs, respectively, were proved to be germline chimeras. The percentage of germline transmission to the donor-derived gametes in the chimeras that received cultured and noncultured gPGCs were 1.9 and 2.2–4.7%, respectively. In conclusion, quail gPGCs retrieved from 5-day-old embryos were thus transmitted in the germline after their transfer to quail embryos of a different strain. This property of the gPGCs was not adversely affected by culture for up to 3 days.
ISSN
0093-691X
Language
English
URI
http://hdl.handle.net/10371/100205
DOI
https://doi.org/10.1016/j.theriogenology.2004.04.014
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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