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An alternative method of deriving embryonic stem cell-like clones by aggregation of diploid cells with tetraploid embryos
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Meeyoung | - |
dc.contributor.author | Jang, Mi | - |
dc.contributor.author | Lee, Eun Ju | - |
dc.contributor.author | Han, Jae Yong | - |
dc.contributor.author | Lim, Jeong Mook | - |
dc.date.accessioned | 2017-01-23T09:00:59Z | - |
dc.date.available | 2017-01-23T09:00:59Z | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | Fertility and Sterility, vol.85 no.1, pp. 1103-1110 | ko_KR |
dc.identifier.issn | 0015-0282 | - |
dc.identifier.uri | https://hdl.handle.net/10371/100223 | - |
dc.description.abstract | Objective: To assess whether embryonic stem (ES) cells could be derived from the aggregation of diploid cells
with tetraploid embryos. Design: Randomized, prospective study. Setting: University embryology and gamete biotechnology laboratory. Animal(s): F1 (C57BL6/DBA2) mice. Intervention(s): Four- to eight-cell F1 tetraploid embryos were aggregated with 10 to 15 donor E14 ES cells. Main Outcome Measure(s): Embryogenesis and ES cell establishment. Result(s): No difference (78% to 89%) in blastocyst formation was detected between the aggregated tetraploid and the control diploid embryos. In a total of 27 transfers, pregnancy was detected in three tetraploid (23.1%) and five diploid (35.7%) cases, and three live births developed from the aggregated tetraploid embryos. The tetraploid blastocysts without aggregation were plated, but no ES cell-like colony was formed. Six of eight aggregated blastocysts derived well-proliferated colonies, which were positive for anti–stage-specific embryonic antigen (SSEA)-1 antibody, Oct-4, and alkaline phosphatase. The microsatellite assay confirmed the homogenous makeup among the donor E14 cells and live-birth and ES-like cells derived from the E14-aggregated, tetraploid embryo. Conclusion(s): The aggregation of pluripotent diploid cells with tetraploid embryos yielded live births and ES-like cells that were homogenous to the donor diploid cells. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | Elsevier | ko_KR |
dc.subject | Mouse embryo | ko_KR |
dc.subject | tetraploidy | ko_KR |
dc.subject | aggregation | ko_KR |
dc.subject | embryonic stem (ES) cell | ko_KR |
dc.subject | characterization | ko_KR |
dc.title | An alternative method of deriving embryonic stem cell-like clones by aggregation of diploid cells with tetraploid embryos | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 조미영 | - |
dc.contributor.AlternativeAuthor | 장미 | - |
dc.contributor.AlternativeAuthor | 이은주 | - |
dc.contributor.AlternativeAuthor | 한재용 | - |
dc.contributor.AlternativeAuthor | 임정묵 | - |
dc.identifier.doi | 10.024 | - |
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