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A testis-mediated germline chimera production based on transfer of chicken testicular cells directly into heterologous testes

Cited 45 time in Web of Science Cited 47 time in Scopus
Authors
Lee, Young Mok; Jung, Jin Gyoung; Kim, Jin Nam; Park, Tae Sub; Kim, Tae Min; Shin, Sang Su; Kang, Dae Kyung; Lim, Jeong Mook; Han, Jae Yong
Issue Date
2006
Publisher
Society for the Study of Reproduction
Citation
Biology of Reproduction, vol.75 no.3, pp. 380-386
Keywords
chickenchimeragamete biologygermlinemale reproductive tractspermatogenesistesticular cellstestis
Abstract
In this study, we proposed a testis-mediated germline chimera production system based on the transplantation of testicular cells directly into heterologous testes. The testicular cells of juvenile (4-wk-old) or adult (24-wk-old) Korean Ogol chickens with a recessive pigmentation inhibitory gene, with or without prior culture, were injected (2 107 cells/head) into the seminiferous tubules of juvenile or adult recipients with White Leghorn with a dominant pigmentation inhibitory gene in a 2×2 factorial arrangement. The localization of transplanted cells into the inner space of the seminiferous tubules was confirmed within 24 h after injection. Subsequent testcross analyses showed that 7.8% (5/64) of the recipients had chimeric status in their testes. The periods of time from transfer to hatching of the first progeny with black feathers were 38 and 45 days for adult cells transplanted into an adult recipient, 188 days for adult cells into a juvenile recipient, and 137 days for juvenile cells into a juvenile recipient. Culture of the testicular cells derived both colony-forming and monolayer-forming cells. The colony-forming cells were stained positively for periodic acid Schiff solution, and further reacted with anti-SSEA-1, anti-SSEA-3, and anti-SSEA-4 antibodies both before and after culture for 15 days. In conclusion, it may be possible to develop the testismediated germline chimera production technique, which extends the feasibility of genetic manipulations in avian species.
ISSN
0006-3363
Language
English
URI
http://hdl.handle.net/10371/100228
DOI
https://doi.org/10.1095/biolreprod.106.052084
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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