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Identification and gene expression profiling of the Pum1 and Pum2 members of the Pumilio family in the chicken

Cited 11 time in Web of Science Cited 10 time in Scopus
Authors
Lee, Jee Young; Lim, Jeong Mook; Kim, Duk Kyung; Zheng, Ying Hui; Moon, Sunjin; Han, Beom Ku; Song, Ki Duk; Kim, Heebal; Han, Jae Yong
Issue Date
2008
Publisher
Wiley
Citation
Molecular Reproduction and Development, vol.75 no.1, pp. 184-190
Keywords
chickenpumiliogene expressionRNA-binding protein
Abstract
Members of the Pumilio (Pum) family of RNA-binding proteins act as translational repressors and are required for germ cell development and asymmetric division. We identified the chicken Pum1 and Pum2 genes and analyzed their expression patterns in various tissues. Comparative sequence analysis of the Pum1 and Pum2 proteins from the drosophila, chicken, mouse, and human revealed a high degree of evolutionary conservation in terms of the levels of homology of the peptide sequences and the structure of Pumilio homology domain (PUM-HD), Cterminal RNA-binding domain, with similar spacing between the adjacent Pum eight tandem repeats. In addition, phylogenetic patterns of pumilio family showed that Pum 1 and 2 of chicken are more closely related to those of mouse and human than other species and Pum1 is more conserved than Pum2. Using real-time RT-PCR, the expression levels of the Pum1 and Pum2 genes were found to be highest in hatched female gonads, and high-level expression of Pum2 was detected in 12-day and hatched gonads among the various chicken embryonic tissues tested. In adult tissues, the expression levels of Pum1 and Pum2 were expressed at higher levels in the testis and muscle than in any other tissue. The characteristics of the tissue-specific expression of Pum genes suggest that Pum1 and Pum2 have effects crucially in particular
stage during development of chicken gonads depending on sexual maturation.
ISSN
1040-452X
Language
English
URI
http://hdl.handle.net/10371/100254
DOI
https://doi.org/10.1002/mrd.20765
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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