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Generation of transgenic quail through germ cell-mediated germline transmission

Cited 53 time in Web of Science Cited 51 time in Scopus
Authors
Shin, Sang Su; Kim, Tae Min; Kim, Sun Young; Kim, Tae Wan; Seo, Hee Won; Lee, Seul Ki; Kwon, Se Chang; Lee, Gwan Sun; Kim, Heebal; Lim, Jeong Mook; Han, Jae Yong
Issue Date
2008
Publisher
Federation of American Society of Experimental Biology
Citation
FASEB Journal, vol.22 no.7, pp. 2435-2444
Keywords
primordial germ cellslentiviral vectoravian transgenesis
Abstract
Here, we describe the production of transgenic quail via a germline transmission system using postmigratory gonadal primordial germ cells (gPGCs). gPGCs retrieved from the embryonic gonads of 5-day-old birds were transduced with a lentiviral vector and subsequently transferred into recipient embryos. Testcross and genetic analyses revealed that among three germline chimeric G0 quail, one male produced transgenic offspring; of 310 hatchlings from the transgenic germline chimera, 24 were identified as donor-derived offspring, and 6 were transgenic (6/310, 1.9%). Conventional transgenesis using stage X blastodermal embryos was also conducted, but the efficiency of transgenesis was similar between the two systems (<1.6 vs. 1.9% for the conventional and gPGC-mediated systems, respectively). However, substantial advantages can be gained from gPGC-mediated method in that it enables an induced germline modification, whereas direct retroviral transfer to stage X embryos causes mosaic integration. The use of gonadal PGCs for transgenesis may lead to the production of bioreactors.
ISSN
0892-6638
Language
English
URI
http://hdl.handle.net/10371/100257
DOI
https://doi.org/10.1096/fj.07-101485
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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