S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Food and Animal Biotechnology (식품·동물생명공학부) Journal Papers (저널논문_식품·동물생명공학부)
Cloning and functional characterization of chicken interleukin-17D
- Hong, Yeong Ho; Lillehoj, Hyun S.; Park, Dong Woon; Lee, Sung Hyen; Han, Jae Yong; Shin, Ji Hye; Park, Myeung Sun; Kim, Jin-Kyoo
- Issue Date
- Veterinary Immunology and Immunopathology, vol.126 no.1-2, pp. 1-8
- The chicken interleukin-17D was cloned from a testis cDNA library prepared from the Korean native chicken. The full-length
chicken IL-17D (chIL-17D) cDNA consisted of a 348 nucleotide sequence encoding an open reading frame of 116 amino acids with
a predicted molecular mass of 13.3 kDa. Comparison of the deduced amino acid sequence of chIL-17D with homologous proteins
from human, mouse and opossum revealed 64%, 53% and 76% identity, respectively, including six conserved cysteine residues
present in the mammalian polypeptides. The chIL-17D gene transcript was expressed in a wide range of tissues, and highest levels
were in pancreas, thymus and lung. Following Eimeria maxima infection, levels of the chIL-17D mRNA were up-regulated in the
intestinal jejunum, bursa, lung, and spleen but decreased in the thymus. Infected chickens also expressed greater levels of chIL-17D
mRNA in CD4+, CD8+ and TCR1+ intestinal intraepithelial lymphocytes while decreased expression was seen in TCR2+ cells.
Treatment of CHCC-OU2 fibroblasts with chIL-17D recombinant protein induced the expression of IL-6 and IL-8. Collectively,
these results suggest that chL-17D has structural and functional similarities to mammalian IL-17Ds and that it plays an important
role in local gut innate immune responses during experimental coccidiosis.
- Files in This Item: There are no files associated with this item.