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Enhanced production of 2,3-butanediol by engineered Saccharomyces cerevisiae through fine-tuning of pyruvate decarboxylase and NADH oxidase activities
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jin-Woo | - |
dc.contributor.author | Kim, Jungyeon | - |
dc.contributor.author | Seo, Seung-Oh | - |
dc.contributor.author | Kim, Kyoung Heon | - |
dc.contributor.author | Jin, Yong-Su | - |
dc.contributor.author | Seo, Jin-Ho | - |
dc.date.accessioned | 2017-02-10T02:02:42Z | - |
dc.date.available | 2017-03-16T16:42:43Z | - |
dc.date.issued | 2016-12-09 | - |
dc.identifier.citation | Biotechnology for Biofuels, 9(1):265 | ko_KR |
dc.identifier.uri | https://hdl.handle.net/10371/100674 | - |
dc.description | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License
(http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. | ko_KR |
dc.description.abstract | Abstract
Background 2,3-Butanediol (2,3-BD) is a promising compound for various applications in chemical, cosmetic, and agricultural industries. Pyruvate decarboxylase (Pdc)-deficient Saccharomyces cerevisiae is an attractive host strain for producing 2,3-BD because a large amount of pyruvate could be shunted to 2,3-BD production instead of ethanol synthesis. However, 2,3-BD yield, productivity, and titer by engineered yeast were inferior to native bacterial producers because of the following metabolic limitations. First, the Pdc-deficient yeast showed growth defect due to a shortage of C2-compounds. Second, redox imbalance during the 2,3-BD production led to glycerol formation that lowered the yield. Results To overcome these problems, the expression levels of Pdc from a Crabtree-negative yeast were optimized in S. cerevisiae. Specifically, Candida tropicalis PDC1 (CtPDC1) was used to minimize the production of ethanol but maximize cell growth and 2,3-BD productivity. As a result, productivity of the BD5_G1CtPDC1 strain expressing an optimal level of Pdc was 2.3 folds higher than that of the control strain in flask cultivation. Through a fed-batch fermentation, 121.8g/L 2,3-BD was produced in 80h. NADH oxidase from Lactococcus lactis (noxE) was additionally expressed in the engineered yeast with an optimal activity of Pdc. The fed-batch fermentation with the optimized 2-stage aeration control led to production of 154.3g/L 2,3-BD in 78h. The overall yield of 2,3-BD was 0.404g 2,3-BD/g glucose which corresponds to 80.7% of theoretical yield. Conclusions A massive metabolic shift in the engineered S. cerevisiae (BD5_G1CtPDC1_nox) expressing NADH oxidase was observed, suggesting that redox imbalance was a major bottleneck for efficient production of 2,3-BD by engineered yeast. Maximum 2,3-BD titer in this study was close to the highest among the reported microbial production studies. The results demonstrate that resolving both C2-compound limitation and redox imbalance is critical to increase 2,3-BD production in the Pdc-deficient S. cerevisiae. Our strategy to express fine-tuned PDC and noxE could be applicable not only to 2,3-BD production, but also other chemical production systems using Pdc-deficient S. cerevisiae. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | BioMed Central | ko_KR |
dc.subject | Pyruvate decarboxylase | ko_KR |
dc.subject | Saccharomyces cerevisiae | ko_KR |
dc.subject | 2, 3-Butanediol | ko_KR |
dc.subject | NADH oxidase | ko_KR |
dc.subject | Metabolomics | ko_KR |
dc.subject | Metabolic engineering | ko_KR |
dc.title | Enhanced production of 2,3-butanediol by engineered Saccharomyces cerevisiae through fine-tuning of pyruvate decarboxylase and NADH oxidase activities | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 김진우 | - |
dc.contributor.AlternativeAuthor | 김정연 | - |
dc.contributor.AlternativeAuthor | 서승오 | - |
dc.contributor.AlternativeAuthor | 김경헌 | - |
dc.contributor.AlternativeAuthor | 진용수 | - |
dc.contributor.AlternativeAuthor | 서진호 | - |
dc.identifier.doi | 10.1186/s13068-016-0677-9 | - |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s) | - |
dc.date.updated | 2017-01-06T10:25:26Z | - |
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