Respiratory syncytial virus infection induces matrix metalloproteinase-9 expression in epithelial cells

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Yeo, S.-J.; Yun, Y.-J.; Lyu, M.-A.; Woo, S.-Y.; Woo, E.-R.; Kim, S.-J.; Lee, H.-J.; Park, H.-K.; Kook, Y.-H.
Issue Date
Springer Verlag
Arch Virol 147: 229-242
Epithelial Cells/*metabolism/virologyGiant Cells/physiologyMatrix Metalloproteinase 9/antagonists & inhibitors/genetics/*metabolismRespiratory Syncytial Virus Infections/*virologyRespiratory Syncytial Viruses/*pathogenicity/physiologyTissue Inhibitor of Metalloproteinase-1/genetics/metabolismTransfectionTumor Cells, CulturedVirus Replication
Increased gelatinolytic activity was observed in respiratory syncytial virus (RSV)-infected HEp-2 cells by using zymography. The anti-matrix metalloproteinase-9 (MMP-9) antibody specifically reduced the gelatinolytic activity suggesting that the increased gelatinolytic activity was due to the MMP-9. It was also supported by the results from immunofluorescent staining, treatment of MMP inhibitors, and RSV infection of the cell clones that were transfected with plasmids to express more MMP-9 and tissue type inhibitor of metalloproteinase-1 (TIMP-1). The gelatinolytic activity of extracellular MMP-9 in RSV-infected HEp-2 cells increased 1.5 +/- 0.2 fold compared with the control (p < 0.01). Cell surface MMP-9 expression was also clearly detected by immunofluorescent staining. Treatment with 1,10-phenanthroline (0.05 mM), ethylenediamine-tetraacetate (EDTA) (1.5 mM), and penta-O-galloyl-beta-D-glucose (PGG) (3.3 microM) inhibited RSV multiplication as well as syncytia formation. Furthermore, the average syncytia size increased when the cells expressing more MMP-9 were infected by RSV. In contrast, syncytia formation was inhibited in the cells manipulated to express TIMP-1. Thus, this study concludes that although RSV infection induces MMP-9, which can enhance the syncytia formation leading to RSV multiplication and spread it can be inhibited by MMP inhibitors.
0304-8608 (Print)
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College of Medicine/School of Medicine (의과대학/대학원)Pediatrics (소아과학전공)Journal Papers (저널논문_소아과학전공)
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