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A novel M cell-specific carbohydrate-targeted mucosal vaccine effectively induces antigen-specific immune responses
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Nochi, Tomonori | - |
dc.contributor.author | Yuki, Yoshikazu | - |
dc.contributor.author | Matsumura, Akiko | - |
dc.contributor.author | Mejima, Mio | - |
dc.contributor.author | Terahara, Kazutaka | - |
dc.contributor.author | Kim, Dong-Young | - |
dc.contributor.author | Fukuyama, Satoshi | - |
dc.contributor.author | Iwatsuki-Horimoto, Kiyoko | - |
dc.contributor.author | Kawaoka, Yoshihiro | - |
dc.contributor.author | Kohda, Tomoko | - |
dc.contributor.author | Kozaki, Shunji | - |
dc.contributor.author | Igarashi, Osamu | - |
dc.contributor.author | Kiyono, Hiroshi | - |
dc.date.accessioned | 2009-11-04T22:27:23Z | - |
dc.date.available | 2009-11-04T22:27:23Z | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | J. Exp. Med. 204:2789-2796 | en |
dc.identifier.issn | 1540-9538 (Electronic) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17984304 | - |
dc.identifier.uri | https://hdl.handle.net/10371/11171 | - |
dc.description.abstract | Mucosally ingested and inhaled antigens are taken up by membranous or microfold cells (M cells) in the follicle-associated epithelium of Peyer's patches or nasopharynx-associated lymphoid tissue. We established a novel M cell-specific monoclonal antibody (mAb NKM 16-2-4) as a carrier for M cell-targeted mucosal vaccine. mAb NKM 16-2-4 also reacted with the recently discovered villous M cells, but not with epithelial cells or goblet cells. Oral administration of tetanus toxoid (TT)- or botulinum toxoid (BT)-conjugated NKM 16-2-4, together with the mucosal adjuvant cholera toxin, induced high-level, antigen-specific serum immunoglobulin (Ig) G and mucosal IgA responses. In addition, an oral vaccine formulation of BT-conjugated NKM 16-2-4 induced protective immunity against lethal challenge with botulinum toxin. An epitope analysis of NKM 16-2-4 revealed specificity to an alpha(1,2)-fucose-containing carbohydrate moiety, and reactivity was enhanced under sialic acid-lacking conditions. This suggests that NKM 16-2-4 distinguishes alpha(1,2)-fucosylated M cells from goblet cells containing abundant sialic acids neighboring the alpha(1,2) fucose moiety and from non-alpha(1,2)-fucosylated epithelial cells. The use of NKM 16-2-4 to target vaccine antigens to the M cell-specific carbohydrate moiety is a new strategy for developing highly effective mucosal vaccines. | en |
dc.language.iso | en | - |
dc.publisher | Rockefeller University Press | en |
dc.subject | Antibodies, Monoclonal/*immunology/pharmacokinetics | en |
dc.subject | Antibody Specificity | en |
dc.subject | Antigens/*immunology | en |
dc.subject | Goblet Cells/immunology | en |
dc.subject | Lectins/immunology | en |
dc.subject | Peyer's Patches/immunology | en |
dc.subject | Rats | en |
dc.subject | Rats, Sprague-Dawley | en |
dc.subject | Respiratory Mucosa/*immunology | en |
dc.subject | Vaccines/*immunology/pharmacokinetics | en |
dc.title | A novel M cell-specific carbohydrate-targeted mucosal vaccine effectively induces antigen-specific immune responses | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김동영 | - |
dc.identifier.doi | 10.1084/jem.20070607 | - |
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