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Monitoring transplanted human mesenchymal stem cells in rat and rabbit bladders using molecular magnetic resonance imaging

Cited 31 time in Web of Science Cited 29 time in Scopus
Authors
Song, Yun Seob; Ku, Ja Hyeon
Issue Date
2007-03-16
Publisher
Wiley-Blackwell
Citation
Neurourol Urodyn. 2007;26(4):584-93.
Keywords
Adipocytes/physiologyAnimalsCell Differentiation/physiologyCell Survival/physiologyChondrocytes/physiologyFerric Compounds/diagnostic useHumansMagnetic Resonance Imaging*Mesenchymal Stem Cell TransplantationNanoparticles/diagnostic useOsteocytes/physiologyRabbitsRatsUrinary Bladder/*cytology/*physiology
Abstract
AIMS: This study investigated whether superparamagnetic iron oxide (SPIO)-labeled human mesenchymal stem cells (hMSCs) may be monitored non-invasively by in vivo magnetic resonance (MR) imaging with conventional 1.5-T system examinations in the bladders of rats and rabbits. METHODS: SPIO were transferred to hMSCs, using GenePORTER. After SPIO-labeled hMSCs were transplanted into the animal bladders, serial T2-weighted MR images and histological examinations were performed over a 4-week period. RESULTS: hMSCs loaded with SPIO, compared to unlabeled cells, showed similar viability. SPIO-labeled hMSCs underwent normal chondrogenic, adipogenic, and osteogenic differentiation. For SPIO-labeled hMSCs concentrations that were greater than 1x10(5), in vitro MR images showed a decrease in signal intensity. MR signal intensity at the areas of SPIO-labeled hMSCs in rat and rabbit bladders were decreased and confined locally. After injection of SPIO-labeled hMSCs into the bladder, MR imaging demonstrated that hMSCs could be seen for at least 12 weeks post-injection. The presence of iron was confirmed with Prussian blue staining in histological sections. CONCLUSIONS: Our findings suggest that hMSCs in animal bladders can be monitored non-invasively with conventional MR imaging.
ISSN
0733-2467 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17357122

http://hdl.handle.net/10371/11575
DOI
https://doi.org/10.1002/nau.20351
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College of Medicine/School of Medicine (의과대학/대학원)Urology (비뇨기과학전공)Journal Papers (저널논문_비뇨기과학전공)
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