SHERP

Methods for expansion of human embryonic stem cells

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Authors
Oh, Sun Kyung; Kim, Hee Sun; Park, Yong Bin; Seol, Hye Won; Kim, Yoon Young; Cho, Myung Soo; Ku, Seung Yup; Choi, Young Min; Kim, Dong-Wook; Moon, Shin Yong
Issue Date
2005-04-26
Publisher
AlphaMed Press
Citation
Stem Cells. 2005 May;23(5):605-9.
Keywords
Cell Culture Techniques/*methodsEmbryo, Mammalian/*cytologyHumansStem Cells/*cytology
Abstract
The manipulation of human embryonic stem cells (hESCs) requires refined skills. Here we introduce both mechanical and enzymatic transfer methods for hESCs depending on experimental purpose. We use the mechanical transfer method for maintenance of hESC lines. Although the method is laborious and time-consuming, the technique permits efficient transfer of undifferentiated hESCs and results in similar clump sizes. We implement the enzymatic transfer method when we need the bulk production of cells for various experiments. The enzyme-treated expansion rapidly produces greater amounts of hESCs within a limited time frame. However, the cell clumps vary in size, and there is a probability that both the differentiated and undifferentiated cells will be transferred. In cases in which there are differentiated colonies, the combination of two methods allows mass production of hESCs by excluding differentiated colonies from passage by manual selection before enzyme treatment.
ISSN
1066-5099 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15849167

http://hdl.handle.net/10371/11578
DOI
https://doi.org/10.1634/stemcells.2004-0297
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Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Obstetrics & Gynecology (산부인과전공)Journal Papers (저널논문_산부인과학전공)
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