Magnetofection-based novel gene delivery

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박경표; 이상우; 박경표
Issue Date
The International Institute of Knowledge Management (TIIKM)
The 3rd International Conference on Nanoscience and nanotechnology 2016, p. 40
Magnetofection-based novel gene delivery의약학salivary glandsgene deliverymagneticmesoporous silicairon oxide nanoparticle
Salivary glands can be a good model for gene delivery to treat systemic and oral disease. Non-viral vectors show little concern about the safety and biocompatibility issues but transfection efficiency is extremely lower than that of viral vectors. In this study, we report a magnetic gene transfer system combining extra-dermal magnetic field and Iron oxide-Mesoporous sillica-PEI nanoparticle to overcome those major hurdles of gene transfer to salivary glands. Uniformly constructed iron oxide nanoparticles were encapsulated by mesoporous silica, then chemically conjugated with linear 10kDa PEI(Poly-ethyl-imine) and TAT peptide (MSNNP). MSNNPs were complexed with therapeutic Aquaporin 5-GFP gene in 20:1(w/w) ratio then transferred to HSG cell-line. Under non-flowing condition transfection efficiency of MSNNP combined with magnetic field was 3 times higher than that of Lipofectamine 2000 but under the 60ml/hr flowing condition MSNNP/magnetic field showed 2.7 folds higher transfection efficiency than Lipofectamine 2000. Confocal image of HSG transfected with MSNNP showed distinctive AQP5-GFP protein expressed and located at membrane region. Magnetofection using MSNNP seems to be a practicable and promising system for non-viral gene delivery to salivary glands.
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dental Science(치의과학과)Others_치의과학과
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