Protective effect of uridine on cornea in a rabbit dry eye model

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Oh, Joo Youn; In, Youn Seok; Kim, Mee Kum; Ko, Jung Hwa; Lee, Hyun Ju; Shin, Ki Cheul; Lee, Sang Mok; Wee, Won Ryang; Lee, Jin Hak; Park, Myunggyu
Issue Date
Association for Research in Vision and Ophthalmology
Invest Ophthalmol Vis Sci. 2007 Mar;48(3):1102-9.
AnimalsApoptosis/drug effectsCell CountCell SurvivalCells, CulturedConjunctiva/cytology/drug effectsDisease Models, AnimalDry Eye Syndromes/metabolism/pathology/*prevention & controlEpithelium, Corneal/drug effects/metabolismFibroblasts/drug effects/metabolismFlow CytometryGlycosaminoglycans/metabolismGoblet Cells/cytology/drug effectsHumansHyaluronic Acid/metabolismMaleMatrix Metalloproteinase 9/metabolismNitric Oxide/metabolismOphthalmic Solutions/administration & dosage/toxicityRabbitsUridine/*administration & dosage/toxicityWound Healing/drug effects
PURPOSE: To investigate the effect of uridine on cultured human corneal epithelial cells and keratocytes in vitro and to evaluate whether the application of uridine-containing eye drops could improve ocular surface health in an in vivo dry eye model. METHODS: Uridine was added to cultured epithelial cells (3 x 10(4) cells/well) and keratocytes (1 x 10(4) cells/well) at various concentrations (0.5-50 microM). Cytotoxicity was tested with the use of MTT assay, and the cells were assessed for apoptosis with the use of flow cytometry. Expressions of hyaluronic acid (HA), glycosaminoglycan (GAG), nitric oxide (NO), and matrix metalloproteinase (MMP)-9 were measured. In vivo, the degree of reepithelialization was assessed after topical application of uridine (100 microM) in a rabbit corneal wound model. Changes in tear production and conjunctival goblet cell counts were investigated after instillation of various concentrations of uridine-containing eye drops in a rabbit dry eye model. RESULTS: In vitro, uridine showed no cellular toxicity. It increased the biosynthesis of HA and GAG and reduced MMP-9 levels in cultured corneal epithelial cells and keratocytes. In vivo, uridine enhanced corneal wound healing and significantly increased the number of conjunctival goblet cells in rabbits. CONCLUSIONS: Uridine can restore the health of the ocular surface in a rabbit corneal wound and dry eye model.
0146-0404 (Print)
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College of Medicine/School of Medicine (의과대학/대학원)Ophthalmology (안과학전공)Journal Papers (저널논문_안과학전공)
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