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RECK expression in osteosarcoma: correlation with matrix metalloproteinases activation and tumor invasiveness

Cited 38 time in Web of Science Cited 45 time in Scopus
Authors
Kang, Hyun-Guy; Kim, Han-Soo; Kim, Kap-Jung; Oh, Joo Han; Lee, Mi-Ra; Seol, So Mi; Han, Ilkyu
Issue Date
2007-01-31
Publisher
John Wiley & Sons
Citation
J Orthop Res. 2007 May;25(5):696-702.
Keywords
Bone Neoplasms/enzymology/*pathologyEnzyme Activation/physiologyGene Expression Regulation, NeoplasticHumansMatrix Metalloproteinase 2/*metabolismMatrix Metalloproteinase 9/*metabolismMembrane Glycoproteins/*geneticsNeoplasm InvasivenessOsteosarcoma/enzymology/*pathologyRNA, Messenger/metabolismTransfectionTumor Cells, Cultured
Abstract
Osteosarcoma is a malignant tumor of bone characterized by its high metastatic potential. For the development of metastasis, activation of matrix metalloproteinases (MMPs) is required. A novel MMPs inhibitor, reversion inducing cysteine rich protein with Kazal motifs (RECK), is known to down-regulate MMPs and suppress the invasive and metastatic potential in many tumor-derived cell lines and some types of tumors. The expression of RECK and its role in tumor invasiveness have never been studied in osteosarcoma. We examined RECK mRNA expression and MMPs activation in osteosarcoma using quantitative real time PCR, gelatin zymography, invasion assay, and transfection experiments. RECK was expressed but down-regulated in osteosarcoma cells. Activation of pro-MMP-2 was observed in all samples, whereas activation of MMP-2 and pro-MMP-9 was detected in only 11% and 7% of the samples, respectively. MMP-9 was not activated in any of the samples. The level of RECK expression was inversely correlated with pro-MMP-2 activation, and overexpression of RECK by transfection resulted in decreased pro-MMP-2 activation and reduced tumor invasiveness. These findings suggest that RECK plays an important role in the invasiveness of osteosarcoma.
ISSN
0736-0266 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17262820

http://hdl.handle.net/10371/11888
DOI
https://doi.org/10.1002/jor.20323
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College of Medicine/School of Medicine (의과대학/대학원)Orthopedic Surgery (정형외과학전공)Journal Papers (저널논문_정형외과학전공)
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