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Multifunctional and Biocompatible Nanomaterials Based on Multiple Quantum Dots/Iron Oxide Nanoparticles-Embedded Silica Nanosphere : 다수의 양자점/산화철 나노입자가 담지된 실리카 나노구체를 기반으로 한 다기능성 생체 친화성 나노물질에 관한 연구
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- Authors
- Advisor
- 이윤식
- Major
- 공과대학 화학생물공학부
- Issue Date
- 2016-02
- Publisher
- 서울대학교 대학원
- Keywords
- Multifunctional nanoparticles ; Quantum dot ; Silica nanoparticle ; Superparamagnetic iron oxide nanoparticle ; Multiplexed detection
- Description
- 학위논문 (박사)-- 서울대학교 대학원 : 화학생물공학부, 2016. 2. 이윤식.
- Abstract
- Quantum dots (QDs) and superparamagnetic Fe3O4 nanoparticles (NPs) have garnered considerable attention in biomedical applications. Novel surface modification strategy could enhance the performance of those NPs.
In chapter 1, silica-coated, QD–embedded silica NPs (QD-SiO2 NPs) containing QDs composed of CdSe@CdS@ZnS were prepared and the structure based advantages were compared with single QD having similar QY. These QD-SiO2 NPs exhibited ca. 200 times stronger photoluminescence (PL) than single QDs. Cytotoxicity studies revealed that QD-SiO2 NPs were less toxic than equivalent numbers of silica-free single QDs. The excellence of QD-SiO2 NPs with regard to in vitro applications was illustrated by significantly enhanced fluorescence signals from QD-SiO2 NPs internalized cancer cells and stem cells.
In chapter 2, QD-assembled silica NPs bearing a polydiacetylene (PDA) supramolecules on their surface (PDA-QD-SiO2 NPs) were described. The resulting PDA-QD-SiO2 NPs showed discrete QD photoluminescence for encoding as well as PDA fluorescence for sensing a target without interference or overlap. Under heating stress of the PDA-QD-SiO2 NPs, the color of the PDA changed from blue to red, which also allowed us to observe the fluorescence emitted from red PDA. The mixture of two different PDA-QD-SiO2 NPs, bluePDA-QD-SiO2 NPs not emitting the fluorescence of PDA and redPDA-QD-SiO2 NPs on which stress was brought on to turn on the PDA fluorescence, was effectively imaged and readily distinguished via fluorescence microscopy, showing their potential for label-free and multiplexed detection of target molecules.
In chapter 3, QDs-embedded silica NPs with an Fe3O4 NP core (M-QD-SiO2 NPs) that has dual functionalities were described. The M-QD-SiO2 NPs were mono-dispersed in size and exhibited super-paramagnetic and highly fluorescent properties. Most of the M-QD-SiO2 NPs were naturally internalized into MDA-MB-231 human breast cancer cells, and the NPs containing cells were successfully sorted by utilizing both fluorescence flow cytometry and a magnetic field. The results indicate that the M-QD-SiO2 NPs have great potential for multimodal cell separation.
In chapter 4, double-layered Fe3O4 NPs containing silica nanoparticles (DL M-SiO2 NPs) were fabricated with a silica core and highly packed Fe3O4 NPs layers. The DL M-SiO2 NPs had a superparamagnetic property and efficient accumulation kinetics under an external magnetic field. Moreover, the magnetic field-exposed DL M-SiO2 NPs show quantitative accumulation, whereas single-layered Fe3O4 NPs containing silica nanoparticles (SL M-SiO2 NPs) and silica-coated Fe3O4 NPs produced a saturated plateau before full recovery of the NPs. DL M-SiO2 NPs are promising nanomaterials with a great potential to separate and analyze biomolecules.
- Language
- English
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