Publications
Detailed Information
Development of a New Rose (Rosa hybrida) Cultivar and Single Nucleotide Polymorphism Markers for Cultivar Identification : 새로운 장미 품종과 품종구별을 위한 SNP 분자표지의 개발
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 강병철 | - |
dc.contributor.author | 허문선 | - |
dc.date.accessioned | 2017-07-13T17:40:08Z | - |
dc.date.available | 2017-07-13T17:40:08Z | - |
dc.date.issued | 2017-02 | - |
dc.identifier.other | 000000141654 | - |
dc.identifier.uri | https://hdl.handle.net/10371/121025 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 식물생산과학부, 2017. 2. 강병철. | - |
dc.description.abstract | The main objectives of this study were to develop a new rose cultivar and to develop single nucleotide polymorphism (SNP) markers to identify cultivars. A new cultivar, To Dios having numerous petals was bred as a cut flower type from the progeny of a cross between Vanilla Perfume and Marcia. An orange-colored standard rose cultivar Vanilla Perfume with 48 petals was used as a female plant. A white-colored standard rose cultivar Marcia with 96 petals was used as a male parent. To Dios is a standard type with 128 peach-colored petals per flower. The vase life of To Dios cultivar can be as long as 15 days. To Dios has the functional stomata in both light and dark conditions, and appropriate stomatal closing helped it to maintain water balance. It takes 47 days from pruning to re-blooming and cut flower productivity is about 160 stems /m2 in a year. When temperature was low, the petal numbers of To Dios was increased and the stamens were converted to petals. To Dios was registered as a new cultivar on Korea Seed & Variety Service in 2014. Since variety protection is important, SNP markers to identify new developed rose cultivars including To Dios were developed. The advantage of SNPs was used to develop a method for identification of cultivars of the polyploid Rosa hybrida using genotyping-by-sequencing (GBS). Sequences obtained from GBS libraries of the genomes of 79 roses were aligned to contigs created by de novo assembly, due to lack of rose reference genome. A total of 1,778 SNPs were selected from 13,488 putative SNPs, genetic correlations among rose cultivars were efficiently analyzed by using these SNPs. SNP markers presented in more than 70% of the 79 cultivars were selected and evaluated, both for polymorphism information content values and level of heterozygosity, and then 20 SNP markers were ultimately selected for high-throughput analysis. Of these 20 SNP markers, 4 were successfully converted into markers for DNA chip assays. High resolution melting analysis was carried out to distinguish further rose genotypes, dependent on heterozygosity and polyploidy. As a result, using a 7 SNP marker set, I was able to discriminate 70.9% of the 79 rose cultivars, and 87.5% of the 16 new cultivars including To Dios developed in Goyang City. SNP markers developed in this study will be useful tools for cultivar identification and genetic correlation study in rose cultivars. | - |
dc.description.tableofcontents | GENERAL INTRODUCTION. 1
CHAPTER I. Development and Characteristics of a New Rose (Rosa hybrida) Cultivar To Dios 13 ABSTRACT 14 INTRODUCTION 16 MATERIALS AND METHODS 19 Conventional rose breeding for a new rose cultivar 19 Cross and selection for To Dios cultivar development 19 Selection and naming of To Dios cultivar 20 Effect of temperature conditions on petal number 21 Vase life evaluation and ethylene treatment 22 Stomata measurement. 23 RNA extraction from floral tissue 24 Quantitative RT-PCR (qRT-PCR) 24 Statistical analysis 25 RESULTS 28 Development and characteristics of To Dios 27 Effect of temperatures on petal number. 37 Cultivation of To Dios 38 Vase life of cut rose cultivars associated with petal number 43 Change in the fresh weight of To Dios 44 Water uptake and water balance of To Dios . 44 Physiological characteristics of epidermal cells of To Dios 51 Molecular biological characteristics of To Dios 55 DISCUSSION 59 LITERATURE CITED 64 CHAPTER II. Development of SNP Markers by GBS (Genotyping-by-Sequencing) for Cultivar Identifi-cation in Rose (Rosa hybrida) 68 ABSTRACT 69 INTRODUCTION 71 MATERIALS AND METHODS 75 Plant materials and DNA extraction 75 GBS library preparation and sequencing 78 Identification of SNPs and phylogenetic analysis 78 Conversion to GBS SNP marker for DNA chip assay 79 High resolution melting analysis 79 RESULTS 81 Sequence production and SNP calling by de novo assembly 81 Distinction between modern roses and wild roses by GBS 86 Phylogenetic analysis of 79 modern rose cultivars 86 Development of SNP markers and genotyping 93 Cultivar identification and genetic diversity analysis 100 DISCUSSION 105 LITERATURE CITED 109 ABSTRACT IN KOREAN 116 APPENDIX 118 | - |
dc.format | application/pdf | - |
dc.format.extent | 2854478 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Rosa hybrida | - |
dc.subject | breeding | - |
dc.subject | petal number | - |
dc.subject | vase life | - |
dc.subject | SNP marker | - |
dc.subject | GBS | - |
dc.subject | cultivar identification | - |
dc.subject.ddc | 633 | - |
dc.title | Development of a New Rose (Rosa hybrida) Cultivar and Single Nucleotide Polymorphism Markers for Cultivar Identification | - |
dc.title.alternative | 새로운 장미 품종과 품종구별을 위한 SNP 분자표지의 개발 | - |
dc.type | Thesis | - |
dc.description.degree | Doctor | - |
dc.citation.pages | xi, 124 | - |
dc.contributor.affiliation | 농업생명과학대학 식물생산과학부 | - |
dc.date.awarded | 2017-02 | - |
- Appears in Collections:
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.