서울대학교 중앙도서관
S-Space 소개
My S-Space
로그인이 필요합니다.
Login

S-Space

Publications

Detailed Information

Novel Secondary Metabolites from Marine Sediment-Derived Actinomycetes

DC Field Value Language
dc.contributor.advisor강헌중-
dc.contributor.author양인호-
dc.date.accessioned2017-07-14T00:36:48Z-
dc.date.available2018-01-22-
dc.date.issued2015-02-
dc.identifier.other000000026146-
dc.identifier.urihttps://hdl.handle.net/10371/121219-
dc.description학위논문 (박사)-- 서울대학교 대학원 : 지구환경과학부, 2015. 2. 강헌중.-
dc.description.abstractMarine natural products are emerging source as an attractive drug pipeline based on its enormous biological diversity of origin. Technical advances like scuba diving, manned submarine boosted the interest to the marine natural products three decades ago and there are eight approved marine drugs now. Most of the approved drugs are based on marine natural products isolated from marine invertebrates, but recent study suggest that most of them are produced by microorganisms. In this study, actinomycetes were isolated from marine envirionments and investigated to discover a novel and bioactive marine natural products.
A chemical investigation of the actinomycetes, Nocardiopsis sp. CNQ115 isolated from marine sediment collected off the coast of southern California resulted in the isolation of two novel imidazole natural compounds nocarimidazoles A and B. The chemical structures of nocarimidazoles A and B were determined by interpretation of NMR data analyses. No significant antibacterial or cytotoxic activities were observed. Investigation of the chemical components of a Streptomyces sp. 10A085 isolated from mudflat sediments collected on the southern coast of the Korean peninsula led to isolation of four new compounds, anithiactins A ? D. The chemical structures of anithiactins were determined by interpretation of NMR data analyses, while the chemical structure of anithiactin B was established from a combination of NMR spectroscopic and crystallographic data analysis. Anithiactins A ? C displayed moderate acetylcholinesterase inhibitory activity with no significant cytotoxicity.		-
dc.description.tableofcontentsChapter 1. Marine Natural Products as a New Pharmaceutical Pipeline 1
1.1 History of Natural Products as a Source for Drug Development 1
1.2 Marine Natural Products as a New Pharmaceutical Pipeline 4
1.2.1 Novelty of Marine Natural Products 4
1.2.2 Current Marine Drugs 7
1.2.3 Major Hurdle of Marine Natural Products derived Drug Discovery 11
Chapter 2. Marine Natural Products from Sediment-Derived Actinomycetes 15
2.1 Introduction 15
2.2 Results and Discussion 16
2.2.1 Structure Elucidation of Nocarimidazoles from CNQ115 16
2.2.1.1 Nocarimidazole A (1) 17
2.2.1.2 Nocarimidazole B (2) 21
2.2.2 Bioactivities of Nocarimidazoles A and B 24
2.3 Experimental 25
2.3.1 Instruments and Data Collection 25
2.3.2 Microorganism Isolation and Fermentation 25
2.3.3 Extraction and Compound Isolation 26
2.3.4 Methylation Reactions 26
2.3.5 Bioassay Procedures 27
2.3.5.1 Cytotoxicity Test 27
2.3.5.2 MIC assay 27
2.3.5.3 Acetylcholinesterase Inhibitory Assay 28
Chapter 3. Marine Natural Products from Mudflat-Derived Actinomycetes 29
3.1 Introduction 29
3.2 Results and Discussion 32
3.2.1 Structure Elucidation of Anithiactins 33
3.2.1.1. Anithiactin A (1) 33
3.2.1.2. Anithiactin B (2) 36
3.2.1.3. Anithiactin C (3) 39
3.2.1.4. Anithiactin D (4) 41
3.2.2 Synthesis of Anithiactin A (1) 44
3.2.3 Bioactivities of Anithiactins A-C 45
3.3 Experimental 47
3.3.1 Instruments and Data Collection 47
3.3.2 Microorganism Isolation and Fermentation 47
3.3.3 Extraction and Compound Isolation 48
3.3.4 X-ray Crystallographic Analyses 48
3.3.5 Synthesis Procedures 49
3.3.6 Bioassay Procedures 51
3.3.6.1 Cytotoxicity Test 51
3.3.6.2 Acetylcholinesterase Inhibitory Assay 51
References 53
Appendix A 58
Appendix B 79
한글초록 121		-
dc.formatapplication/pdf-
dc.format.extent8347674 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subjectMarine natural products-
dc.subjectmarine microorganisms-
dc.subjectmarine actinomycetes-
dc.subject2-phenylthiazole-
dc.subject9-
dc.subject11-secosterol-
dc.subject.ddc550-
dc.titleNovel Secondary Metabolites from Marine Sediment-Derived Actinomycetes-
dc.typeThesis-
dc.contributor.AlternativeAuthorInho Yang-
dc.description.degreeDoctor-
dc.citation.pages123-
dc.contributor.affiliation자연과학대학 지구환경과학부-
dc.date.awarded2015-02-
Appears in Collections:
Files in This Item:

Altmetrics

Item View & Download Count

Show Simple Item Record
Find it @ SNU

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share

qrcode
SNS Share