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Detection of JC virus type 1 in peripheral lymphocytes, brain and cerebrospinal fluid from two Korean AIDS patients with progressive multifocal leukoencephalopathy

Cited 4 time in Web of Science Cited 3 time in Scopus
Authors
Jeong, Byung-Hoon; Jin, Jae-Kwang; Choi, Eun-Kyoung; Kim, Kyung-Hoon; Choi, Kang-Won; Oh, Myoung-Don; Shin, Ki-Chul; Kim, Yong-Sun
Issue Date
2002
Publisher
Karger
Citation
Intervirology 2002;45:94-100
Keywords
AIDS-Related Opportunistic Infections/*virologyBase SequenceBrain/*virologyDNA, Viral/analysis/cerebrospinal fluidGenome, ViralGenotypeJC Virus/classification/genetics/*isolation & purificationLeukoencephalopathy, Progressive Multifocal/etiology/pathology/*virologyLymphocytes/*virologyMolecular Sequence DataPhylogenyPolymerase Chain Reaction
Abstract
The human polyomavirus JC virus (JCV) is the etiologic agent of the fatal demyelinating central nervous system disease progressive multifocal leukoencephalopathy (PML), which occurs in 4-7% of AIDS patients. Two Korean AIDS patients with PML were assayed for JCV, and the virus was genotyped by polymerase chain reaction, DNA sequencing and phylogenetic analysis. Using immunohistochemical analysis, we also examined the distribution of JCV antigen in the brains of the patients. The JCV genome was detected in peripheral lymphocytes, brain and cerebrospinal fluid from these Korean PML patients. Although type 2 is the most common genotype in Asia, the genotype of the JCV in these two AIDS patients was characterized as type 1, which is of European origin. We found that JCV antigen was selectively detected in oligodendrocytes and astrocytes of the brains from these patients. Compared to the prototype type 1 (Mad-1), two different nucleotides (G-->C) in the KOR-1 strain identified here were found at positions 2488 and 2490 of the major capsid protein VP1 gene. In summary, this is the first report of PML in Korean AIDS patients; it is also the first isolation of JCV type 1 in PML in East Asians.
ISSN
0300-5526 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12145541

http://hdl.handle.net/10371/12124
DOI
https://doi.org/10.1159/000063229
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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