A 588-gene microarray analysis of the peripheral blood mononuclear cells of spondyloarthropathy patients

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Gu, Wiley-Blackwell; Marker-Hermann, E.; Baeten, D.; Tsai, W. C.; Gladman, D.; Xiong, M.; Deister, H.; Kuipers, J. G.; Huang, F.; Song, Y. W.; Maksymowych, W.; Kalsi, J.; Bannai, M.; Seta, N.; Rihl, M.; Crofford, L. J.; Veys, E.; De Keyser, F.; Yu, D. T. Y.
Issue Date
Oxford University Press
Rheumatology 2002;41:759-66
Antigens, Differentiation/blood/*geneticsArthritis, Psoriatic/blood/geneticsArthritis, Rheumatoid/blood/geneticsChemokine CXCL12Chemokines, CXC/blood/geneticsDNA/analysisGenetic MarkersLeukocytes, Mononuclear/*metabolism*Oligonucleotide Array Sequence AnalysisReceptors, CXCR4/blood/geneticsReverse Transcriptase Polymerase Chain ReactionSpondylitis, Ankylosing/blood/*genetics/pathologySynovial Membrane/metabolism/pathology
OBJECTIVES: To identify genes which are more highly expressed in the peripheral blood mononuclear cells (PBMC) of patients with spondyloarthropathy (SpA), rheumatoid arthritis (RA) and psoriatic arthritis (PsA), in comparison to normal subjects. METHODS: A 588-gene microarray was used as a screening tool to select a panel of such genes from PBMC of these subjects and of normal subjects. Results were then validated by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: The following genes were more highly expressed in arthritis patients than in normal subjects: macrophage differentiation marker MNDA (myeloid nuclear differentiation antigen), MRP8 and MRP14 (migratory inhibitory factor-related proteins); signalling molecules JAK3 (janus kinase 3) and MAP kinase p38 (mitogen-activated protein kinase); receptors TNFR2/p75, C-C-chemokine receptor type 1 (CCR1), C-X-C-chemokine receptor type 4 (CXCR4) and integrin beta1; and the cytokines/chemokines interleukin (IL) 1beta and IL-8. Expression of CXCR4 was unexpectedly high among all arthritis subjects. Using RT-PCR, ELISA and immunohistology, expression of stromal cell-derived factor 1 (SDF-1) was demonstrated in arthritis joints. CONCLUSIONS: The CXCR4/SDF-1 is a potential pro-inflammatory axis for RA, PsA and SpA.
1462-0324 (Print)
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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