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p130 mediates TGF-β-induced cell-cycle arrest in Rb mutant HT-3 cells : p130 mediates TGF-beta-induced cell-cycle arrest in Rb mutant HT-3 cells
DC Field | Value | Language |
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dc.contributor.author | Choi, Hyun Ho | - |
dc.contributor.author | Jong, Hyun-Soon | - |
dc.contributor.author | Song, Sang Hyun | - |
dc.contributor.author | Kim, Tae You | - |
dc.contributor.author | Kim, Noe Kyeong | - |
dc.contributor.author | Bang, Yung-Jue | - |
dc.date.accessioned | 2009-11-13T06:14:38Z | - |
dc.date.available | 2009-11-13T06:14:38Z | - |
dc.date.created | 2020-12-21 | - |
dc.date.issued | 2002-08 | - |
dc.identifier.citation | Gynecologic Oncology, Vol.86 No.2, pp.184-189 | - |
dc.identifier.issn | 0090-8258 | - |
dc.identifier.other | 119298 | - |
dc.identifier.uri | https://hdl.handle.net/10371/12138 | - |
dc.description.abstract | Objective. The retinoblastoma proteins include Rb and the functionally and structurally related proteins p107 and p130. It has been reported that HT-3 cells are sensitive to TGF-beta growth inhibition, despite the Rb mutation. The purpose of this study was to elucidate the growth-inhibitory mechanism of TGF-beta in Rb mutant HT-3 cells. Methods. Growth inhibition by TGF-beta in cervical carcinoma cell lines was evaluated by counting cell numbers. Cell-cycle distribution was determined by staining DNA with propidium iodide (PI) and measured using a flow cytometer. The level of each protein expression was determined by Western blot analysis. To evaluate the assembly of cdk2/p21, cdk2/cyclin E, and E2174/p130 complexes by TGF-beta, immunoprecipitation was performed. Results. TGF-beta inhibited the proliferation of HT-3 cells expressing mutant Rb protein and efficiently induced cell-cycle arrest at G, phase. p21 protein level was elevated in TGF-beta-treated HT-3 cells, while other G, regulatory protein levels were unaltered. TGF-beta markedly enhanced the binding of p21 with cdk2 but decreased that of cdk2 with cyclin E and inhibited the phosphorylation of p130 but did not change Rb and p107 protein status. We also found that E2F-1 protein level was lower in TGF-beta-treated cells and suggest that this might be the result of enhanced binding between E2F-4 and p130. Conclusions. Our results demonstrate that p130, instead of Rb, can mediate growth inhibition by TGF-beta in Rb mutant HT-3 cells. (C) 2002 Elsevier Science (USA). | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Academic Press | - |
dc.title | p130 mediates TGF-β-induced cell-cycle arrest in Rb mutant HT-3 cells | - |
dc.title.alternative | p130 mediates TGF-beta-induced cell-cycle arrest in Rb mutant HT-3 cells | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 방영주 | - |
dc.identifier.doi | 10.1006/gyno.2002.6740 | - |
dc.citation.journaltitle | Gynecologic Oncology | - |
dc.identifier.wosid | 000177268000014 | - |
dc.identifier.scopusid | 2-s2.0-0036351215 | - |
dc.citation.endpage | 189 | - |
dc.citation.number | 2 | - |
dc.citation.startpage | 184 | - |
dc.citation.volume | 86 | - |
dc.identifier.sci | 000177268000014 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Tae You | - |
dc.contributor.affiliatedAuthor | Kim, Noe Kyeong | - |
dc.contributor.affiliatedAuthor | Bang, Yung-Jue | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | GROWTH-FACTOR-BETA | - |
dc.subject.keywordPlus | RETINOBLASTOMA PROTEIN | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | P107 | - |
dc.subject.keywordPlus | PRB | - |
dc.subject.keywordPlus | E2F | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | LINES | - |
dc.subject.keywordAuthor | transforming growth factor-beta | - |
dc.subject.keywordAuthor | retinoblastoma protein family | - |
dc.subject.keywordAuthor | p21 | - |
dc.subject.keywordAuthor | cervical carcinoma | - |
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