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Apoptotic cell death of human colon cancer cell lines after extracellular calgranulin B uptake : 대장암세포주의 외래 Calgranulin B 단백질 포식에 의한 세포사멸
DC Field | Value | Language |
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dc.contributor.advisor | 구자록 | - |
dc.contributor.author | 김근 | - |
dc.date.accessioned | 2017-07-14T01:15:28Z | - |
dc.date.available | 2017-07-14T01:15:28Z | - |
dc.date.issued | 2015-08 | - |
dc.identifier.other | 000000066656 | - |
dc.identifier.uri | https://hdl.handle.net/10371/121774 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 협동과정 종양생물학전공, 2015. 8. 구자록. | - |
dc.description.abstract | The molecular mechanisms through which extracellular calgranulin B affects colon cancer cells remain unclear. My present study focused on the systemic link between calgranulin B and colon cancer. In patients tumor tissues, calgranulin B protein showed a positive correlation with stromal inflammatory cells surrounding tumor cells, and methylation of the gDNA promoter region of calgranulin B was observed in both patients paired tissues and human colon cancer cell lines. Notably, extracellular calgranulin B could penetrate colon cancer cells, but not the other types of cancer cells tested. Internalization of calgranulin B was possible via various mechanisms depending on colon cancer cells and caused apoptotic cell death. In addition, calgranulin B inhibited aurora A kinase activity in a dose-dependent manner and suppressed tumor growth in a mouse model. My findings demonstrate the antitumor effects of calgranulin B in the inflammatory microenvironment of colon cancer. | - |
dc.description.tableofcontents | Abstract i
Contents iii List of tables vi List of figures vii Introduction 1 Materials and Methods 9 Human cancer cell lines 9 Western blot analysis 9 gDNA PCR 10 Bisulfite-modified sequencing analysis 11 Immunohistochemistry of tumor tissues and statistical analysis 12 Confocal microscopy 14 Flow cytometry 15 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay 15 Cell cycle analysis 16 Terminal transferase dUTP nick end labelling assay 17 In vivo study 17 18F-FDG positron emission tomography?computed tomography imaging 18 Immunohistochemistry in the xenograft model 19 Construction of Glutathione S-Transferase (GST)-human calgranulin B fusion protein 19 Human protein microarray 21 Immunoprecipitation 21 Aurora A kinase assay 22 Results 24 Calgranulin B was not expressed in colon cancer cell lines. 24 Tissue calgranulin B level in tumor cells was correlated with that of stromal inflammatory cells around tumor glands in colon cancer. 29 Internalization of extracellular calgranulin B into colon cancer cells was mediated by various pathways depending on cell type. 32 Extracellular treatment of calgranulin B induced antitumor effects in colon cancer cells. 37 Intravenous injection of calgranulin B suppressed tumor volume in the HCT-116 colon cancer xenograft model. 42 Calgranulin B interacted with aurora A kinase and the activity of aurora A kinase decreased in the presence of calgranulin B. 45 Discussion 54 References 62 Abstract in Korean 77 | - |
dc.format | application/pdf | - |
dc.format.extent | 2082424 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | calgranulin B | - |
dc.subject | colon cancer | - |
dc.subject | protein internalization | - |
dc.subject | inflammatory microenvironment | - |
dc.subject | aurora A kinase | - |
dc.subject.ddc | 616 | - |
dc.title | Apoptotic cell death of human colon cancer cell lines after extracellular calgranulin B uptake | - |
dc.title.alternative | 대장암세포주의 외래 Calgranulin B 단백질 포식에 의한 세포사멸 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Kun Kim | - |
dc.description.degree | Doctor | - |
dc.citation.pages | vii, 79 | - |
dc.contributor.affiliation | 의과대학 협동과정 종양생물학전공 | - |
dc.date.awarded | 2015-08 | - |
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