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Expression and role of interleukin-6 in distraction osteogenesis

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dc.contributor.authorCho, T.-J.-
dc.contributor.authorKim, J. A.-
dc.contributor.authorChung, C. Y.-
dc.contributor.authorYoo, W. J.-
dc.contributor.authorGerstenfeld, L. C.-
dc.contributor.authorEinhorn, T. A.-
dc.contributor.authorChoi, I. H.-
dc.date.accessioned2009-11-16T06:32:39Z-
dc.date.available2009-11-16T06:32:39Z-
dc.date.issued2007-03-07-
dc.identifier.citationCalcif Tissue Int 80:192-200en
dc.identifier.issn0171-967X (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17340223-
dc.identifier.urihttp://hdl.handle.net/10371/12210-
dc.description.abstractDistraction osteogenesis is a special form of bone healing in which well-controlled distraction stresses and consequent tensile strains within callus tissue induce very efficient new bone formation. Proinflammatory cytokines are involved during the early phase of fracture healing and callus remodeling. Temporal expression patterns of proinflammatory cytokines were assessed in Sprague-Dawley rat tibial models of distraction osteogenesis and acute lengthening, and only interleukin-6 (IL-6) was found to be specifically induced during the distraction phase. IL-6 immunoreactivity was detected not only in hemopoietic cells and osteoblasts but also in the spindle-shaped cells of the fibrous interzone, where most of the tensile strains are concentrated. In vitro study revealed that IL-6 did not affect the proliferation of C3H10T1/2 cells, mouse bone marrow stromal cells (MSCs), or MC3T3-E1 cells; but its blocking antibody reduced the proliferation of C3H10T1/2 cells and MSCs. The mRNA expression of COL1A1 and osteopontin were not changed by IL-6 or its blocking antibody, but the alkaline phosphatase activities of MC3T3-E1 cells were increased by IL-6 and decreased by its blocking antibody. These findings indicate that IL-6 is a proinflammatory cytokine that responds to tensile strain during distraction osteogenesis. IL-6 negatively affects the proliferation of primitive mesenchymal cells, whereas the differentiation of more mature osteoblastic lineage cells is enhanced by IL-6 in vitro. IL-6 appears to be one of the cytokines involved in the complex network of signal cascades evoked during distraction osteogenesis and may differentially affect immature and mature osteoblastic lineage cells.en
dc.language.isoen-
dc.publisherSpringer Verlagen
dc.subject3T3 Cellsen
dc.subjectAnimalsen
dc.subjectCell Lineen
dc.subjectCell Proliferationen
dc.subjectCytokines/metabolismen
dc.subjectDose-Response Relationship, Drugen
dc.subjectInflammationen
dc.subjectInterleukin-6/*biosynthesis/*physiologyen
dc.subjectMiceen
dc.subjectMice, Inbred C3Hen
dc.subjectOsteoblasts/metabolismen
dc.subjectOsteogenesisen
dc.subjectRats, Sprague-Dawleyen
dc.titleExpression and role of interleukin-6 in distraction osteogenesisen
dc.typeArticleen
dc.identifier.doi10.1007/s00223-006-0240-y-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Orthopedic Surgery (정형외과학전공)Journal Papers (저널논문_정형외과학전공)
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