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Radiation-induced esophagitis in vivo and in vitro reveals that epidermal growth factor (EGF) is a potential candidate for therapeutic intervention strategy : 방사선유발 식도염의 in vivo, in vitro 모델에서 표피성장인자(epidermal growth factor)의 효능 연구
DC Field | Value | Language |
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dc.contributor.advisor | 김학재 | - |
dc.contributor.author | 김경수 | - |
dc.date.accessioned | 2017-07-14T01:36:37Z | - |
dc.date.available | 2017-07-14T01:36:37Z | - |
dc.date.issued | 2016-08 | - |
dc.identifier.other | 000000136030 | - |
dc.identifier.uri | https://hdl.handle.net/10371/122155 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 의학과 방사선종양학과, 2016. 8. 김학재. | - |
dc.description.abstract | Purpose: To establish and characterize radiation-induced esophagitis (RIE) in vivo and in vitro.
Materials and Methods: Fractionated thoracic irradiation at 0, 8, 12, or 15 Gy was given daily for 5 days to Balb/c or C57Bl/6 mice. Changes in the body weight gain and daily food intake were assessed. At the end of the study, we harvested the esophagus and examined: (i) histology by H&E staining | - |
dc.description.abstract | (ii) immune cell infiltration and apoptosis by Fluorescent activated cell sorting (FACS) | - |
dc.description.abstract | (iii) gene expression changes by qRT-PCR. Het-1A human esophageal epithelial cells were irradiated at 6 Gy, treated with recombinant human growth factors, and examined for gene expression changes, apoptosis, proliferation, and signal transduction pathways.
Results: We observed that irradiation at 12 Gy or 15 Gy per fraction produced a significant body weight reduction and decreased food intake in Balb/c mice, but not so much in C57Bl/6 mice. Further analyses of Balb/c mice irradiated at 12 Gy/fraction revealed an attenuated epithelium, inflamed mucosa, and increased number of infiltrating CD4+ helper T cells and apoptotic cells. Moreover we found that expressions of tissue inhibitor for metalloproteinase-1, plasminogen activator inhibitor-1, granulocyte macrophage-colony stimulating factor, vascular endothelial growth factor, and stromal-derived factor-1 were increased while epidermal growth factor (Egf) was decreased. Irradiated Het-1A cells similarly showed a significant decrease in EGF and connective tissue growth factor (CTGF) expression. Treatment of EGF but not CTGF partially protected Het-1A cells from radiation-induced apoptosis and revealed phosphorylation of EGFR, AKT and ERK signaling pathways. Conclusions: We established a mouse model of RIE in Balb/c mice with 12 Gy × 5 fractions, which exhibited reduced body weight gain, food intake, and histopathologic features similar to human esophagitis. Decreased EGF expression in the irradiated esophagus suggests that EGF may be a potential therapeutic intervention strategy to treat RIE. | - |
dc.description.tableofcontents | Introduction 1
Materials and Methods 4 Results 10 Discussion 24 References 30 국문 초록 38 | - |
dc.format | application/pdf | - |
dc.format.extent | 964815 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | ko | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Radiation-induced esophagitis | - |
dc.subject | mouse model | - |
dc.subject | epidermal growth factor | - |
dc.subject.ddc | 610 | - |
dc.title | Radiation-induced esophagitis in vivo and in vitro reveals that epidermal growth factor (EGF) is a potential candidate for therapeutic intervention strategy | - |
dc.title.alternative | 방사선유발 식도염의 in vivo, in vitro 모델에서 표피성장인자(epidermal growth factor)의 효능 연구 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Kyung Su Kim | - |
dc.description.degree | Doctor | - |
dc.citation.pages | 40 | - |
dc.contributor.affiliation | 의과대학 의학과 | - |
dc.date.awarded | 2016-08 | - |
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