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Extracellular-matrix cryogels for cartilage tissue engineering : 연골조직공학을위한세포외기질과합성/천연고분자를응용한크라이오젤합성

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dc.contributor.advisor황석연-
dc.contributor.author한민의-
dc.date.accessioned2017-07-14T02:23:52Z-
dc.date.available2017-07-14T02:23:52Z-
dc.date.issued2016-02-
dc.identifier.other000000132956-
dc.identifier.urihttps://hdl.handle.net/10371/122459-
dc.description학위논문 (석사)-- 서울대학교 대학원 : 협동과정 바이오엔지니어링 전공, 2016. 2. 황석연.-
dc.description.abstractIn this thesis, we investigated highly porous extracellular matrix (ECM) cryogels for cartilage tissue engineering. For ECM cryogel fabrication, methacrylated chondroitin sulfate (MeCS) or methacrylated hyaluronic acid (MeHA) was co-polymerized with PEG-based or gelatin-based system via free radial initiation under freezing condition. This procedure induced ice crystal formation resulting in macroporous structures within ECM-cryogels. The developed PEG-based ECM-cryogel system exhibited average macroporosity of 75% and supported the chondrocyte infiltration. Additionally, gelatin-based ECM cryogel showed average porosity of 65%. When rabbit chondrocytes were cultured on PEG-based ECM cryogels, PEG/CS cryogels stimulated aggrecan gene expression and GAG accumulation, whereas PEG/HA cryogels stimulated type II collagen gene expression and collagen accumulation. Presence of CS in gelatin-based cryogels showed strong upregulation in all types of cartilage related genes (type II collagen, type X collagen, aggrecan, HAS2, PRG4, Link protein). Gelatin/HA cryogel showed significant increase in HAS2, link protein and type II collagen. These results demonstrated that the ECM cryogels can be utilized to promote specific ECM secretion for cartilage tissue engineering-
dc.description.tableofcontentsCHAPTER ONE: THE SCIENTIFIC BACKGROUND AND RESEARCH PROGRESS 7
1.1 Cartilage tissue engineering 7
1.2 Extracellular matrix (ECM) components for scaffold 7
1.3 Cryogels 9
1.4 Aims of research 11

CHAPTER TWO: Poly (ethylene glycol) diacrylate- based cryogel for cartilage tissue engineering 12
2.1 Introduction 12
2.2 Materials and methods 12
2.2.1 Isolation and culture of rabbit chondrocytes 12
2.2.2 Methacrylation of chondroitin sulfate and hyaluronic acid 13
2.2.3 Fabrication of PEGDA based cryogels 14
2.2.4 Rheological and mechanical analysis 14
2.2.5 Swelling ratios analysis 15
2.2.6 Scanning electron microscopy analysis 15
2.2.7 Live/dead assay 16
2.2.8 Biochemical assay 16
2.2.9 Real-time pcr analysis 17
2.2.10 In vivo transplantation 17
2.2.11 Histological analysis 18
2.2.12 Immunohistochemical staining anlaysis with aggrecan and collagen type II 18
2.2.13 Statistical analysis 19
2.3 Results& Discussion 19
2.3.1 Preparation and characterization of cryogels 19
2.3.2 In vitro cell viability on cryogels 27
2.3.3 ECM-specified cartilage matrix synthesis 28
2.3.4 ECM-specified cartilage gene expressions 30
2.3.5 in vitro histological analysis 33
2.3.6 In vivo cartilage formation 34
2.4 Conclusion 36

CHAPTER THREE: Gelatin-based cryogel for cartilage tissue engineering 38
3.1 Introduction 39
3.2 Materials and methods 39
3.2.1 Synthesis of methacrylate gelatin 39
3.2.2 Fabrication of gelatin cryogels 39
3.2.3 Physical characterization of gelatin based cryogels 40
3.2.4 Statistical analysis 40
3.3 Results& discussion 41
3.3.1 Preparation and characterization of gelatin-based cryogels 41
3.3.2 In vitro cell viability on gelatin cryogels 46
3.3.3 Effect of ECM-based cryogels on chondrocyte ECM synthesis 47
3.3.4 Gene expression analysis of chondrocytes in gelatin-based cryogels 49
3.3.5 In vitro histological analysis 51
3.3.6 In vivo histological analysis 52
3.4 Conclusion 54

REFERENCE 55

초록(국문요약) 61		-
dc.formatapplication/pdf-
dc.format.extent2853554 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subjectCryogel-
dc.subjectHyaluronic acid-
dc.subjectChondroitin sulfate-
dc.subjectCartilage Tissue engineering-
dc.subjectPoly (ethylene glycol) diacrylate-
dc.subjectGelatin-
dc.subject.ddc660-
dc.titleExtracellular-matrix cryogels for cartilage tissue engineering-
dc.title.alternative연골조직공학을위한세포외기질과합성/천연고분자를응용한크라이오젤합성-
dc.typeThesis-
dc.description.degreeMaster-
dc.citation.pages62-
dc.contributor.affiliation공과대학 협동과정 바이오엔지니어링전공-
dc.date.awarded2016-02-
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