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Transcription factor gene fgzc248 is involved in DNA damage response in Fusarium graminearum : Fusarium graminearum의 전사조절인자 FgZC248의 기능연구
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 이인원 교수님 | - |
| dc.contributor.author | 부민민 | - |
| dc.date.accessioned | 2017-07-14T06:43:12Z | - |
| dc.date.available | 2017-07-14T06:43:12Z | - |
| dc.date.issued | 2014-08 | - |
| dc.identifier.other | 000000021924 | - |
| dc.identifier.uri | https://hdl.handle.net/10371/125879 | - |
| dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 농생명공학부, 2014. 8. 이인원. | - |
| dc.description.abstract | Fusarium graminearum is an important plant pathogen which causes Fusarium head blight in cereal crops. The maintenance of genome integrity plays important role in the survival or virulence of the plant pathogen since DNA acts as the carrier of genetic information. Transcription factors (TFs) orchestrate gene expression under the control of various cellular signaling pathways and therefore function as key mediators of cellular functions. To identify TFs related to DNA damage responses in F. graminearum, four different DNA damage agents were used to screen the previously generated F. graminearum transcription factor mutant library. Sixteen transcription factor gene deletion mutants showed altered sensitivity to at least one DNA damage agent. Among them, the fgzc248 deletion mutant was specifically sensitive to hydroxyurea than the wild-type strain. Moreover, deletion of fgzc248 resulted in defects in virulence and sexual development. Result of the alkaline comet assay demonstrated that disruption of fgzc248 function resulted in DNA damages in cells. Levels of transcript accumulation of DNA repair genes increased in the fgzc248 deletion mutant compared with wild-type. This study is the first characterizing a novel transcription factor FgZC248 involved in DNA damage response and will provide new insights in mechanisms underlying DNA damage responses in F. graminearum. | - |
| dc.description.tableofcontents | Abstract
CONTENTS I. Introduction II. Materials and methods 1. Strains and culture conditions 2. Nucleic acid manipulations, PCR primers and conditions 3. Genetic manipulations and Fungal transformations 4. Sexual crosses 5. Virulence test 6. Quantitative real-time PCR (qRT)-PCR 7. Alkaline comet assay 8. RNA-seq analysis III. Results 1. Screening of transcription factor gene deletion mutants in F. graminearum 2. Identification of novel transcription factor gene fgzc248 in F. graminearum 3. Deletion and complementation 4. Phenotypic analyses 5. Virulence analysis 6. Accumulation of DNA damage in cells 7. Effects of fgzc248 on regulation of genome-wide transcript levels IV. Discussion V. Reference VI. Abstract in Korean LIST OF TABLES Table 1 DNA damage agents used in the study Table 2 Fungal transformation primers used in this study Table 3 Primers used in qRT-PCR Table 4 Putative TFs involved in DNA damage responses Table 5 Changes in transcription levels between Δfgzc248 and wild-type strain in functional categories Table 6 Transcript levels of selected genes involved in DNA damage and cell cycle LIST OF FIGURES Fig. 1 Transcription factor gene deletion mutants involved in DNA damage responses in F. graminearum Fig. 2 Identification of FgZC248 and its distribution in fungi Fig. 3 Targeted deletion and complementation of the fgzc248 Fig. 4 Mycelial growth of wild-type, Δfgzc248 mutant, and complementation strains on CM and CM supplemented with 10 mM HU Fig. 5 Growth rate and perithecium formation of wild-type, Δfgzc248 mutant, and complementation strains on carrot agar Fig. 6 Virulence of wild-type, Δfgzc248 mutant, and complementation strains on wheat head Fig. 7 Detection of increased DNA damage in Δfgzc248 strain and HU treated cells | - |
| dc.format | application/pdf | - |
| dc.format.extent | 1192319 bytes | - |
| dc.format.medium | application/pdf | - |
| dc.language.iso | en | - |
| dc.publisher | 서울대학교 대학원 | - |
| dc.subject | DNA damage response | - |
| dc.subject | Fusarium graminearum | - |
| dc.subject | transcription factor | - |
| dc.subject | hydroxyurea | - |
| dc.subject.ddc | 630 | - |
| dc.title | Transcription factor gene fgzc248 is involved in DNA damage response in Fusarium graminearum | - |
| dc.title.alternative | Fusarium graminearum의 전사조절인자 FgZC248의 기능연구 | - |
| dc.type | Thesis | - |
| dc.description.degree | Master | - |
| dc.citation.pages | 48 | - |
| dc.contributor.affiliation | 농업생명과학대학 농생명공학부 | - |
| dc.date.awarded | 2014-08 | - |
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