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Orobol, an Enzyme-Converted Product of Genistein, Suppresses Obesity by Targeting Casein Kinase 1 epsilon : Casein Kinase 1 epsilon 활성 저해를 통한 오로볼의 비만 억제 효능
DC Field | Value | Language |
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dc.contributor.advisor | 이기원 | - |
dc.contributor.author | Hae Ji | - |
dc.date.accessioned | 2017-07-14T06:46:09Z | - |
dc.date.available | 2018-03-30 | - |
dc.date.issued | 2016-02 | - |
dc.identifier.other | 000000133313 | - |
dc.identifier.uri | https://hdl.handle.net/10371/125939 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 농생명공학부, 2016. 2. 이기원. | - |
dc.description.abstract | Obesity is one of the most important risk factors in the various diseases including type 2 diabetes, cardiovascular diseases, and multiple forms of cancers. Due to side-effects of anti-obesity drugs, natural materials have been studied to be used as alternatives for obesity treatment. There have been many evidences that isoflavones derived from soybean play a beneficial role in obesity. In the present study, the anti-obesity effect of orobol which is one of soy isoflavones has been investigated in adipogenic cocktail (MDI)-induced 3T3-L1 adipocytes and high-fat diet (HFD)-induced male C57BL/6J obese mice model. During MDI-induced adipogenesis in 3T3-L1 pre-adipocytes, orobol 20 M suppressed lipid accumulation and decreased protein expression levels of peroxisome proliferator-activated receptor-γ (PPARγ), CCAT/ enhancer-binding protein-α (C/EBPα) and fatty acid synthase (FAS). Orobol blocked adipogenesis from early stage to terminal differentiation by inhibiting Casein Kinase 1 epsilon (CK1ε) and its downstream signaling pathways, eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) in 3T3-L1 preadipocytes. Also orobol treatment resulted in reduced lipid accumulation in HFD-fed mice model. In summary, I firstly identified orobol significantly suppressed adipogenesis and this inhibitory effect exerted through CK1ε/4E-BP1 in 3T3-L1 preadipocytes. These findings suggest orobol can be a novel therapeutic agent to treat obesity. | - |
dc.description.tableofcontents | Ⅰ. Introduction 1
Ⅱ. Materials and methods 5 2.1. Reagents 5 2.2. Cell culture and preadipocytes differentiation 6 2.3. Cell proliferation assay 7 2.4. Oil Red O staining 8 2.5. Western blot assay 9 2.6. Kinase assay 10 2.7. Pull-down assay 11 2.8. Animal study 12 2.9. Statistical analysis 13 Ⅲ. Results 14 3.1. Orobol inhibits MDI-induced adipogenesis in 3T3-L1 preadipocyte 14 3.2. Orobol blocks MDI-induced lipid accumulation through all stages of adipogenesis in 3T3-L1 preadipocytes 19 3.3. Orobol inhibits CK1ε kinase activity 22 3.4. Orobol suppresses 4E-BP1 phosphorylation in 3T3-L1 preadipocytes 25 3.5. Orobol ameliorates obesity in HFD-fed mice 28 Ⅳ. Discussion 32 Ⅴ. References 36 Ⅵ. 국문 초록 44 | - |
dc.format | application/pdf | - |
dc.format.extent | 729538 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Orobol | - |
dc.subject | 5 | - |
dc.subject | 7 | - |
dc.subject | 3’ | - |
dc.subject | 4’-Tetrahydroxyisoflavone | - |
dc.subject | Obesity | - |
dc.subject | Adipogenesis | - |
dc.subject | CK1 | - |
dc.subject | 4E-BP1 | - |
dc.subject | 3T3-L1 preadipocytes | - |
dc.subject | C57BL/6J mice | - |
dc.subject.ddc | 630 | - |
dc.title | Orobol, an Enzyme-Converted Product of Genistein, Suppresses Obesity by Targeting Casein Kinase 1 epsilon | - |
dc.title.alternative | Casein Kinase 1 epsilon 활성 저해를 통한 오로볼의 비만 억제 효능 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | 지해 | - |
dc.description.degree | Master | - |
dc.citation.pages | vi, 46 | - |
dc.contributor.affiliation | 농업생명과학대학 농생명공학부 | - |
dc.date.awarded | 2016-02 | - |
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