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The effect of fermented buckwheat on producing L-carnitine and Gamma-aminobutyric acid (GABA) enriched designer eggs
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 김도만 | - |
dc.contributor.author | 박남현 | - |
dc.date.accessioned | 2017-07-17T08:54:58Z | - |
dc.date.available | 2018-07-04T02:22:54Z | - |
dc.date.issued | 2017-02 | - |
dc.identifier.other | 000000141800 | - |
dc.identifier.uri | https://hdl.handle.net/10371/127132 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 국제농업기술학과, 2017. 2. 김도만. | - |
dc.description.abstract | The potential of fermented buckwheat as feed additives was studied to increase L-carnitine and gamma-aminobutyric acid (GABA) in designer eggs. Buckwheat contains high levels of lysine, methionine, and glutamate, which are precursors for the synthesis of L-carnitine and GABA. Rhizopus oligosporus was used for the fermentation of buckwheat to produce L-carnitine and GABA that exert positive effects like enhanced metabolism, antioxidant activities, immunity, and blood pressure control.
A novel analytical method for simultaneously detecting L-carnitine and GABA was developed using LC/MS and LC/MS/MS. The fermented buckwheat extract contained 4 and 34 fold-increased L-carnitine and GABA, respectively, than normal buckwheat. Compared to control, the fermented buckwheat extract-fed group showed enriched L-carnitine (13.6%) and GABA (8.4%) in the yolk. However, only L-carnitine was significantly different (p<0.05). Egg production (9.4%), albumen weight (2.1%), and shell weight (5.8%) were significantly increased (p<0.05). There was no significant difference in yolk weight, total cholesterol (1.9%) and triglyceride (4.9%) in the yolk was lowered (p<0.05). Fermented buckwheat as feed additives has the potential to produce L-carnitine and GABA enriched designer eggs with enhanced nutrition and homeostasis. The L-carnitine and GABA enriched designer eggs pose significance to be utilized in superfood production and supplement industries. | - |
dc.description.tableofcontents | I. INTRODUCTION 1
II. MATERIALS AND METHODS 6 1. Microbial strain and culture condition 6 2. Preparation of fermented buckwheat 6 3. Analysis of the fermented buckwheat 7 4. The feeding experiment with Hy-Line brown hens 11 5. Analyses of the designer egg 13 6. Statistical analysis 15 III. RESULTS 16 1. Analysis of L-carnitine and GABA by modified LC/MS or LC/MS/MS 16 2. The amount of L-carnitine and GABA in fermented buckwheat was increased 19 3. Daily egg production was increased with fermented buckwheat feed 19 4. Weights of albumen and egg shell were increased with fermented buckwheat feed 20 5. The amount of total cholesterol and triglyceride in yolk with fermented buckwheat feed showed slight decrease 23 6. The concentrations of L-carnitine and GABA in egg yolk were increased by feeding fermented buckwheat 26 IV. DISCUSSION 28 V. Conclusion 37 VI. REFERENCES 39 VII. 국문초록 (Abstract) 45 | - |
dc.format | application/pdf | - |
dc.format.extent | 603550 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Buckwheat | - |
dc.subject | Fermentation | - |
dc.subject | Rhizopus oligosporus | - |
dc.subject | L-carnitine | - |
dc.subject | Gamma-aminobutyric acid | - |
dc.subject | Designer Egg | - |
dc.subject.ddc | 631 | - |
dc.title | The effect of fermented buckwheat on producing L-carnitine and Gamma-aminobutyric acid (GABA) enriched designer eggs | - |
dc.type | Thesis | - |
dc.description.degree | Master | - |
dc.citation.pages | 46 | - |
dc.contributor.affiliation | 국제농업기술대학원 국제농업기술학과 | - |
dc.date.awarded | 2017-02 | - |
dc.embargo.terms | 2017-08-01 | - |
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