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Fabrication of Polypyrrole Nanoparticles via Different Polymerization Methods and Their Cytotoxicity Evaluation : 다양한 제조방법을 통한 폴리피롤 나노 입자의 합성과 세포 독성 평가
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 장정식 | - |
dc.contributor.author | 김세나 | - |
dc.date.accessioned | 2017-07-19T05:57:00Z | - |
dc.date.available | 2017-07-19T05:57:00Z | - |
dc.date.issued | 2016-02 | - |
dc.identifier.other | 000000132211 | - |
dc.identifier.uri | https://hdl.handle.net/10371/129405 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 화학생물공학부, 2016. 2. 장정식. | - |
dc.description.abstract | The cellular effect of polypyrrole (PPy) nanoparticles with similar geometry (size and shape) was scrutinized by cellular uptake and in vitro cytotoxicity assessments in human lung epithelial A549 cell and human embryonic kidney epithelial HEK293 cells. PPy1~4 nanoparticles with a diameter of ca. 50 nm and spherical shape were successively fabricated by four different polymerization methods, two dispersion polymerization and two microemulsion polymerization. Cellular uptake of PPy1~4 nanoparticles in both cell lines was evaluated by transmission electron microscopy. Cytotoxic effects were systemically investigated using cell viability, reactive oxygen species production, apoptosis/necrosis measurement, and live-cell microscopy. PPy3 nanoparticles using dodecyltrimetylammonium bromide exhibited the highest levels of toxicity in both cell lines, because of the cellular effects of unique physicochemical properties. In addition, viability in both cell lines was concentration- and cell-dependent with the nanoparticles. These findings may provide the methods-dependent nanotoxicity and uptake of PPy nanoparticles, which could be useful in their application toward biomedical and bioelectronics fields. | - |
dc.description.tableofcontents | Chapter 1. Introduction 1
1.1 Cytotoxicity of nanomaterials 1 1.2 Conducting polymer nanomaterials 4 1.3 Fabrication of nanomaterials 6 1.4 Physicochemical properties of nanomaterials 10 1.5 Objective of this study 14 Chapter 2. Experimental 15 2.1 Materials 15 2.2 Fabrication of PPy nanoparticles 15 2.3 Characterization of PPy nanoparticles 17 2.4 Cell culture 18 2.5 Transmission electron microscopy observation 18 2.6 Cell viability assay 19 2.7 Reactive oxygen species (ROS) 20 2.8 Measurement of mitochondrial superoxide 21 2.9 Quantification of apoptotic and necrotic cells 21 2.10 Live cell observation of apoptosis and necrosis in cells 22 Chapter 3. Results and discussion 23 3.1 Characterization of PPy nanoparticles 23 3.2 Cellular uptake of PPy nanoparticles 32 3.3 Cell viability 35 3.4 Reactive oxyzen species 38 3.5 Apoptosis and necrosis 42 Chapter 4. Conclusion 47 References 49 국문초록 55 | - |
dc.format | application/pdf | - |
dc.format.extent | 2279949 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Polypyrrole nanoparticle | - |
dc.subject | Polymerization method | - |
dc.subject | Physicochemical properties | - |
dc.subject | Cell viability | - |
dc.subject | Cytotoxicity | - |
dc.subject | Reactive oxygen species | - |
dc.subject.ddc | 660 | - |
dc.title | Fabrication of Polypyrrole Nanoparticles via Different Polymerization Methods and Their Cytotoxicity Evaluation | - |
dc.title.alternative | 다양한 제조방법을 통한 폴리피롤 나노 입자의 합성과 세포 독성 평가 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Seh-na Kim | - |
dc.description.degree | Master | - |
dc.citation.pages | 56 | - |
dc.contributor.affiliation | 공과대학 화학생물공학부 | - |
dc.date.awarded | 2016-02 | - |
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