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Effects of RhoA/ROCK inhibitors on chondrogenesis of human periodontal ligament-derived mesenchymal stem cells
DC Field | Value | Language |
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dc.contributor.advisor | 조재진 | - |
dc.contributor.author | 김설희 | - |
dc.date.accessioned | 2017-07-19T08:31:39Z | - |
dc.date.available | 2017-07-19T08:31:39Z | - |
dc.date.issued | 2014-02 | - |
dc.identifier.other | 000000018461 | - |
dc.identifier.uri | https://hdl.handle.net/10371/131072 | - |
dc.description | 학위논문 (석사)-- 서울대학교 치의학대학원 : 치의학과, 2014. 2. 조재진. | - |
dc.description.abstract | Several researches reported that the control of cytoskeleton activity via RhoA-ROCK may promote the chondrogenic differentiation of stem cells. Therefore, we determined whether the inhibition of RhoA-ROCK pathway by ROCK inhibitor Y-27632 and actin polymerization inhibitor cytochalasin D can promote chondrogenesis in PDLSCs in the presence or absence of TGF-β3. PDLSCs were isolated and purified from the periodontal ligament of the human third molar teeth. After initiating chondrogenesis with 3D cell cluster formation, the clusters were maintained under 10 ng/mL TGF-β3, 3 μM cytochalasin D, 10 μM Y-27632, TGF-β3+cytochalasin D, TGF-β3+Y-27632 and without treatment as negative control. We analyzed the chondro-clusters by glycosaminoglycan assay (GAG), histology evaluation, safranin O and von Kossa staining and PCR. Immunohistochemistry were performed to measeure the expression for Collagen I, II as well as aggrecan. Clusters treated with TGF-β3 and Y-27632 had the highest level of GAG synthesis. Unlike cytochalasin D, Y-27632 was found to exert a synergistic effect as well. Combination treatment of TGF-β3 and Y-27632 increased the expression of the chodro-related genes, collagen type II and sox9 while decreasing expression of the osteogenic gene, Runx2. On the other hand, treatment with TGF-β3 and cytochalasin D let do the greatest expression of Runx2 and collagen X, a hypertrophic chondrocyte marker gene. Calcium deposits were visualized by von Kossa staining. The least amount of calcium deposition was found in clusters exposed to TGF-β3 with Y-27632. In conclusion, our data suggest that the treatment of TGF-β3 along with RhoA/ROCK inhibition using Y-27632 induced more selective chondrogenesis. Furthermore, PDLSCs and control of the RhoA/ROCK pathway have the potential to be for cartilage tissue repair including the temporomandibular joint disc regeneration. | - |
dc.description.tableofcontents | 목 차
Introduction 1 Materials and methods 4 Results 8 Discussion 13 참고문헌 25 초 록 31 그림 목차 [그림 1] 19 [그림 2] 20 [그림 3] 21 [그림 4] 22 [그림 5] 23 [그림 6] 24 | - |
dc.format | application/pdf | - |
dc.format.extent | 785092 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | periodontal ligament | - |
dc.subject | mesenchymal stem cell | - |
dc.subject | RhoA/ROCK | - |
dc.subject | Y-27632 | - |
dc.subject | chondrogenesis | - |
dc.subject.ddc | 617 | - |
dc.title | Effects of RhoA/ROCK inhibitors on chondrogenesis of human periodontal ligament-derived mesenchymal stem cells | - |
dc.type | Thesis | - |
dc.description.degree | Master | - |
dc.citation.pages | ⅳ, 32 | - |
dc.contributor.affiliation | 치과대학 치의학과 | - |
dc.date.awarded | 2014-02 | - |
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