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Effect of maternal stress on circadian rhythm and metabolism
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 김경진 | - |
dc.contributor.author | 윤성식 | - |
dc.date.accessioned | 2017-07-19T09:03:56Z | - |
dc.date.available | 2017-07-19T09:03:56Z | - |
dc.date.issued | 2013-02 | - |
dc.identifier.other | 000000008290 | - |
dc.identifier.uri | https://hdl.handle.net/10371/131540 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 생명과학부, 2013. 2. 김경진. | - |
dc.description.abstract | It is well established that maternal stress causes defective functions in several important brain regions such as hypothalamus, hippocampus and amygdala. However, the effect of maternal stress on the suprachiasmatic nucleus (SCN) which acts as a central pacemaker driving circadian rhythm remains largely unknown. In the present study, I focused on the question whether stress in pregnant mice could influence the circadian clockwork in the SCN of their offspring and its physiological relevance. SCN slices from 1 week-old transgenic mice maintained rhythmic PER1::LUC expression for more than a week, and maternal stress caused shortened periodicity and reduced amplitude, and even loss of rhythm in some cases. However, in SCN slices from 5 week-old mice, altered period and arrhythmicity were recovered as similar to those of adult mice while the amplitude of PER1::LUC was still lower than the control group. In addition, mRNA profiles of several molecular clock genes in liver of prenatally stressed adult mice were affected. In accordance with the liver clock, rhythmic profiles of metabolic genes involved in glucose metabolism were altered in maternally stressed mice. However, although liver glycogen contents were slightly decreased, plasma glucose levels were not changed, suggesting that maternal stress cannot disrupt glucose homeostasis in normal feeding condition. Taken together, these findings suggest that maternally stressed mice can adjust to their environment after birth, likely for overcoming the effect of prenatal stress on circadian timing system | - |
dc.description.tableofcontents | ABSTRACT………………………………………………………………………… 1
INTRODUCTION………………………………………………………………….… 3 MATERIALS AND METHODS…………………………………………………..… 8 Animal care and handling Maternal stress procedure. Preparation of plasma and tissues RNA isolation and RT-PCR Measurement of metabolic parameters Ex vivo tissue explant culture Quantitative real-time bioluminescence monitoring on the SCN Statistical analysis RESULTS……………………………………………………………………………15 Altered cyclic PER1::LUC expression in SCN from 1 week-old mice Cyclic PER1::LUC expression in SCN from 5 week-old mice Expression of clock genes in the local clock Effect of maternal stress on carbohydrate metabolism DISCUSSION………………………………………………………………………. 33 REFERENCES……………………………………………………………………... 37 ABSTRACT IN KOREAN…………………………………………………………. 44 | - |
dc.format | application/pdf | - |
dc.format.extent | 759348 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject.ddc | 570 | - |
dc.title | Effect of maternal stress on circadian rhythm and metabolism | - |
dc.type | Thesis | - |
dc.description.degree | Master | - |
dc.citation.pages | 44 | - |
dc.contributor.affiliation | 자연과학대학 생명과학부 | - |
dc.date.awarded | 2013-02 | - |
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