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Studies on expression of eIF2α kinases in the mouse male germ cells : 생쥐 웅성생식세포에서 eIF2α kinases 발현연구
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 이건수 | - |
dc.contributor.author | 박교선 | - |
dc.date.accessioned | 2017-07-19T09:03:59Z | - |
dc.date.available | 2017-07-19T09:03:59Z | - |
dc.date.issued | 2013-02 | - |
dc.identifier.other | 000000008798 | - |
dc.identifier.uri | https://hdl.handle.net/10371/131541 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 생명과학부, 2013. 2. 이건수. | - |
dc.description.abstract | The mammalian male germ cells should be maintained below the core body temperature for their successful development. A previous study in my laboratory reported that eIF2α (α subunit of eukaryotic translation initiation factor 2) phosphorylation is one of immediate responses of male germ cells against heat stress. In this work, the expression of eIF2α protein was observed in all stages of male germ cells. However, phospho-eIF2α was detected only at cytoplasm of germ cell stages up to early pachytene spermatocytes by heat treatment. In order to identify a candidate kinase responsible for eIF2α phosphorylation upon heat stress in male germ cells, I examined expression of the four known eIF2α kinases (HRI, PKR, PERK, and GCN2) in mouse testis. The results showed that all four eIF2α kinases are expressed with distinct developmental stage-specific manners. Furthermore, a phosphorylated form of PERK is detected in the testis from heat-treated mouse. These results suggest that PERK is the candidate kinase that phosphorylates eIF2α by sensing heat stress in the mouse male germ cells. | - |
dc.description.tableofcontents | INTRODUCTION 1
MATERIALS AND METHODS 5 Animals and heat treatment 5 Reverse transcription and quantitative real-time PCR 5 Antibodies 6 Immunohistochemistry 6 Immunoblot analysis 7 RESULTS 9 Developmental stage-specific phosphorylation of eIF2α by heat stress in male mouse germ cells 9 Expression levels of eIF2α kinases in mouse testis 10 Developmental stage-specificity of eIF2α kinases expression in male mouse germ cells 12 Phosphorylation of PERK in heat-stressed mouse testis 14 ER stress induction by heat stress in mouse testis 15 DISCUSSION 36 REFERENCES 40 ABSTRACTS IN KOREAN 50 | - |
dc.format | application/pdf | - |
dc.format.extent | 1436682 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.title | Studies on expression of eIF2α kinases in the mouse male germ cells | - |
dc.title.alternative | 생쥐 웅성생식세포에서 eIF2α kinases 발현연구 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Kyosun Park | - |
dc.description.degree | Master | - |
dc.citation.pages | 51 | - |
dc.contributor.affiliation | 자연과학대학 생명과학부 | - |
dc.date.awarded | 2013-02 | - |
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