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17-Oxo-Docosahexaenoic acid induces Nrf2-mediated expression of heme oxygenase-1 and NAD(P)H:quinone oxidoreductase in mouse skin
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 서영준 | - |
dc.contributor.author | 우스만 | - |
dc.date.accessioned | 2017-07-19T11:08:54Z | - |
dc.date.available | 2017-07-19T11:08:54Z | - |
dc.date.issued | 2016-02 | - |
dc.identifier.other | 000000132930 | - |
dc.identifier.uri | https://hdl.handle.net/10371/133395 | - |
dc.description | 학위논문 (석사)-- 서울대학교 융합과학기술대학원 : 융합과학기술대학원 분자의학 및 바이오제약학과, 2016. 2. 서영준. | - |
dc.description.abstract | Dietary omega-3 polyunsaturated fatty acids (PUFAs) have been shown to have therapeutic and preventive potential in the management of oxidative stress- and inflammation-associated ailments, such as diabetes mellitus, neurodegenerative disorders and various types of cancers. Docosahexaenoic acid (DHA), a representative omega-3 PUFA, is capable of activating multiple anti-oxidant and cytoprotective signaling pathways. Nrf2 plays a key role in cellular stress responses. In the present study, the effects of DHA on Nrf2-mediated expression of anti-oxidative enzymes were examined in mouse skin. Topical application of DHA significantly activated Nrf2 and upregulated expression of its target proteins, namely heme oxygenase-1 (HO-1) and NAD(P)H:quinone oxidoreductase (NQO-1). According to the recent studies, DHA undergoes metabolism to produce electrophilic oxo-derivatives that modulate several cellular redox signaling pathways more efficiently than the parent compound. One such metabolite is 17-oxo-DHA. Topical application of 17-oxo-DHA markedly elevated the expression of Nrf2-mediated expression of HO-1 and NQO-1 in hairless mouse skin apparently by stimulating the degradation of cytosolic Keap1, a negative regulator of Nrf2. Notably, 17-oxo-DHA even at a lower dose (20 nmol), robustly upregulated the expression of aforementioned cytoprotective proteins as compared to DHA (10 µmol). In contrast to 17-oxo-DHA, 17-Hydroxy-DHA lacking the α,β-unsaturated carbonyl moiety was a much weaker inducer of Nrf2 activation and its target protein expression. | - |
dc.description.tableofcontents | Introduction 1
Materials and Methods 3 Results 8 Discussion 23 References 28 | - |
dc.format | application/pdf | - |
dc.format.extent | 1536464 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 융합과학기술대학원 | - |
dc.subject | Mouse skin | - |
dc.subject | Nrf2 | - |
dc.subject | Keap1 | - |
dc.subject | DHA | - |
dc.subject | 17-oxo-DHA | - |
dc.subject | cytoprotective proteins | - |
dc.subject.ddc | 610 | - |
dc.title | 17-Oxo-Docosahexaenoic acid induces Nrf2-mediated expression of heme oxygenase-1 and NAD(P)H:quinone oxidoreductase in mouse skin | - |
dc.type | Thesis | - |
dc.description.degree | Master | - |
dc.citation.pages | 36 | - |
dc.contributor.affiliation | 융합과학기술대학원 분자의학 및 바이오제약학과 | - |
dc.date.awarded | 2016-02 | - |
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