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Characterization of a complete genome of a circular single-stranded DNA virus from porcine stools in Korea
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 박봉균 | - |
| dc.contributor.author | 김아름 | - |
| dc.date.accessioned | 2017-07-19T11:30:16Z | - |
| dc.date.available | 2017-07-19T11:30:16Z | - |
| dc.date.issued | 2015-02 | - |
| dc.identifier.other | 000000024761 | - |
| dc.identifier.uri | https://hdl.handle.net/10371/133721 | - |
| dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 수의학과, 2015. 2. 박봉균. | - |
| dc.description.abstract | Porcine circular single-stranded DNA viruses have been just identified from swine feces in Korea. This virus was mentioned as bovine stool-associated circular DNA virus (BoSCV)-like virus discovered from porcine stools. However, the thorough characteristics of the virus were not identified. Therefore, this research focuses on finding a full genome sequence and analyzing the genetic features of the virus. The virus, now called porcine stool-associated circular DNA virus in Korea (PoSCV Kor), consists of 2,589 bases forming circular structure. It has two major ORFs inversely encoding replicase and capsid protein, with each stem-loop structure between 5' ends and 3' ends of the two putative ORFs. This characteristics is the same as PoSCV in New Zealand, but different from chimpanzee stool-associated circular virus (ChiSCVs) and BoSCV, which have one stem-loop structure. Therefore, it would be sure that PoSCV Kor is very similar to PoSCV in respect to the genetic aspect | - |
| dc.description.abstract | the same number of nucleotide bases and high amino acid identity of replicase and capsid protein (96 and 93%, respectively). This fact could be certified through the finding that PoSCV Kor and PoSCV are in the same cluster by phylogenetic analysis based on the comparison with full-sequences of other circular ssDNA viruses. | - |
| dc.description.tableofcontents | ABSTRACT
CONTENTS LIST of TABLES LIST of FIGURES INTRODUCTION MATERIALS AND METHODS Porcine samples DNA extraction Nested PCR and detection of the existence of the virus PCR for full-sequencing TA cloning for sequencing Phylogenetic analysis and protein fuction prediction Nucleotide sequence accession number RESULTS Detection and sequence analysis of circular DNA genome Screening of stool DNA samples for PoSCV Kor Phylogenetic analysis of PoSCV Kor Genome organization of PoSCV Kora Estimates of evolutionary divergence between partial replicase sequence Nucleotide and amino acid sequence identity in the partial replicase region DISCUSSION REFERENCES 국문초록 | - |
| dc.format | application/pdf | - |
| dc.format.extent | 609223 bytes | - |
| dc.format.medium | application/pdf | - |
| dc.language.iso | en | - |
| dc.publisher | 서울대학교 대학원 | - |
| dc.subject | PoSCV | - |
| dc.subject | complete genome | - |
| dc.subject.ddc | 636 | - |
| dc.title | Characterization of a complete genome of a circular single-stranded DNA virus from porcine stools in Korea | - |
| dc.type | Thesis | - |
| dc.description.degree | Master | - |
| dc.citation.pages | 28 | - |
| dc.contributor.affiliation | 수의과대학 수의학과 | - |
| dc.date.awarded | 2015-02 | - |
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