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Strain Specific Detection and Quantification of Probiotic Bifidobacterium bifidum BGN4 in Mouse Model and Positive Effect of B. bifidum BGN4 on Intestinal Microflora
Bifidobacterium bifidum BGN4 특이 검출을 통한 장관 내 균주 정량 및 장내 균 총 개선효과

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Authors
전수정
Advisor
지근억
Major
생활과학대학 식품영양학과
Issue Date
2017
Publisher
서울대학교 대학원
Keywords
Bifidobacterium bifidum BGN4polymerase chain reactioncolonization
Description
학위논문 (석사)-- 서울대학교 대학원 : 식품영양학과, 2017. 2. 지근억.
Abstract
Bifidobacterium species have been used in the production of functional foods based on their well-known beneficial effects on intestinal health. B. bifidum BGN4 is a commercialized probiotic strain with proven evidence in clinical trials but still lacks a DNA-based identification method. This study aimed to develop a specific polymerase chain reaction (PCR) primer set for strain specific identification of B. bifidum BGN4 using whole genome sequences and applied research. Consequently, a primer set targeting 200 bp was developed. The developed primer was applied to detect and quantify B. bifidum BGN4 strain in the fecal sample after feeding ICR mouse and bio-distribution of this strain was analyzed at each position of the intestinal tract. Strong viability of B. bifidum BGN4 was identified by analysis of gastrointestinal contents and mouse fecal samples. Also, intake of B. bifidum BGN4 promoted the quantity and viability of Bifidobacterium genus in GI tract. This study revealed that the administration of B. bifidum BGN4 promoted the colonization of not only B. bifidum BGN4 but also the other Bifidobacterium strains.
Language
English
URI
http://hdl.handle.net/10371/133988
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College of Human Ecology (생활과학대학)Dept. of Food and Nutrition (식품영양학과)Theses (Master's Degree_식품영양학과)
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