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MALDI-TOF MS Identification of Peptide Citrullination Site Using Br Signature
MALDI-TOF 질량분석에서 브롬 동위원소 패턴을 이용한 펩타이드 시트룰린화 위치 확인에 대한 연구

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Authors
최미경
Advisor
김희준
Major
자연과학대학 화학부
Issue Date
2013-02
Publisher
서울대학교 대학원
Keywords
citrullinationdeiminationcitrulline4-bromophenyl glyoxalMALDI-TOF MS
Description
학위논문 (석사)-- 서울대학교 대학원 : 화학부 분석화학 전공, 2013. 2. 김희준.
Abstract
Enzymatic conversion of arginine to citrulline by peptidyl arginine deiminase (PAD) is associated with peptide presentation and development of autoimmunity in rheumatoid arthritis. In order to facilitate identification of the citrullination site, citrulline residue was modified using 4-bromophenyl glyoxal (BPG), and 194 Da mass increase and incorporation of the Br signature were confirmed by MALDI-TOF MS. Using this approach, we identified four and five citrullination sites of bovine serum albumin (BSA) and bovine fibrinogen, respectively. MALDI-TOF/TOF MS was used to unambiguously identify two citrullination sites from bovine fibrinogen.
Language
Korean
URI
http://hdl.handle.net/10371/134875
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College of Humanities (인문대학)Korean Language and Literature (국어국문학과)Others_국어국문학과
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