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Physico-immunological Characterizations of Exogenous Substances (Palmitoleic Acid and Okadaic Acid) in Bivalves : 이매패류에 있어 외인성 화합물(Palmitoleic Acid와 Okadaic Acid)에 의한 생리면역학적 특성 규명
DC Field | Value | Language |
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dc.contributor.advisor | 박세창 | - |
dc.contributor.author | 치쳉 | - |
dc.date.accessioned | 2017-10-27T17:00:41Z | - |
dc.date.available | 2017-10-27T17:00:41Z | - |
dc.date.issued | 2017-08 | - |
dc.identifier.other | 000000145145 | - |
dc.identifier.uri | https://hdl.handle.net/10371/137012 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 수의과대학 수의학과, 2017. 8. 박세창. | - |
dc.description.abstract | Abstract
Physico-immunological Characterizations of Exogenous Substances (Palmitoleic Acid and Okadaic Acid) in Bivalves Chi Cheng Major in Veterinary Pathobiology and Preventive Medicine Department of Veterinary Medicine The Graduate School of Seoul National University Palmitoleic acid (PA), an algicidal compound, is used against Alexandrium tamarense | - |
dc.description.abstract | however, its impacts on scallops are still unclear. Okadaic acid (OA) is produced by dinoflagellates during harmful algal blooms and is a diarrhetic shellfish poisoning toxin. This study evaluated the effect of harmful algal blooms-related environmental substances on scallops to gain a better understanding of effect of algicide PA and the toxicity of algal toxin OA, and then help improve the intensive breeding and long-term sustainability of scallop farming. Therefore, in the current investigation, various immunological parameters (total hemocyte counts (THC), reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH), lactate dehydrogenase (LDH), and nitric oxide (NO), superoxide dismutase (SOD), acid phosphatase (ACP), alkaline phosphatase (ALP), lysozyme activities, and total protein content) were assessed in the hemolymph of scallops at 3, 6, 12, 24, and 48 h post-exposure (hpe) to different concentrations of PA (20, 40, and 80 mg/L) and OA (50, 100, and 500 nM). Moreover, the expressions of immune-related genes (CLT-6, FREP, HSP90, PGRP, MT, Cu/ZnSOD, MnSOD, PrxV, and BD) were also investigated after exposing bay scallop for 3, 6, 12, 24, and 48 h to any of three different concentrations of PA and OA.
Results showed that the lysozyme activity was decreased in scallop treated with PA at 12, 24 and 48 hpe, as compared to control. Although, the SOD activity was higher in all PA treated groups but the significant increment was observed only in 20 mg/L of PA treated group at 12, 24 and 48 hpe. The ACP activities were enhanced from 6–48 hpe in 80 mg/L of PA treated group, as compared to control. The total protein level was enhanced in all the PA treated groups, especially in 40 mg/L of PA treatment group at 3 hpe and in 80 mg/L of PA treatment group at 6, 12, 24 and 48 hpe. THC decreased in PA-treated groups, whereas ALP increased significantly in all of the PA treatment groups at 3 hpe, after which it significantly decreased. The LDH and NO levels were significantly enhanced in the high and medium concentration group. Notably, the GSH level increased in all PA treatment groups at each time interval. Our study also revealed that after treatment with different concentrations of PA, variable effects on the expression of genes involved in the immune system response were observed. Our research demonstrated that immersing scallops in PA may disrupt the endocrine system or immune responses. Therefore, the present study highlights the potential risk of using the PA as an algicide to control algal bloom outbreaks in the marine environment. Moreover, the current investigation revealed the effects of exposure to OA on the immune responses of bay scallop. Results showed that SOD and ACP activities were decreased between 24–48 hpe. The ALP, lysozyme activities, and total protein levels were also modulated after exposure to different concentrations of OA. The ROS, MDA, and NO levels and LDH activity were enhanced after exposure to different concentrations of OA | - |
dc.description.abstract | however, both THC and GSH decreased between 24–48 hpe. The expression of immune-system-related genes was also assessed at different time points during the exposure period. Therefore, these results provide a better understanding of the response status of bivalves against DSP toxins.
Overall, our investigation suggest that the exposure to algicide PA and algal toxin OA had negative effects on the antioxidant and non-specific immune responses, and even disrupted the metabolism of bay scallops, making them more vulnerable to environmental stress-inducing agents | - |
dc.description.abstract | Key words: Mollusks, Immunological Response, Bay Scallop, Algicide, Algal Toxin, Palmitoleic Acid, Okadaic Acid
Student number: 2014-31493 | - |
dc.description.tableofcontents | General introduction 1
Literature Review 5 A. Mollusks 5 A.1. Bivalve mollusks 5 A.2. Innate immune system of bivalves 6 A.3. Biological costs of adverse environmental changes 7 A.4. Responses to abiotic stress of bivalves 7 A.5. Responses to toxicant stress of bivalves 8 B. Algicide 9 B.1. Algicide classification 9 B.2. Special algicidal bacterium 10 B.3. Bioactive algicidal compound 10 C. Marine toxins 11 C.1. Shellfish poisoning classification 11 C.2. Amnesic shellfish poisoning 12 C.3. Neurotoxic shellfish poisoning 12 C.4. Paralytic shellfish poisoning 12 C.5. Diarrhetic shellfish poisoning 13 C.6. Azaspiracid shellfish poisoning 13 Reference 15 Chapter I Effect of the Algicide Palmitoleic Acid on Immune Functions of Bay Scallop (Argopecten Irradians) 21 Abstract 21 1.1. Introduction 23 1.2. Materials and methods 26 1.3. Results 31 1.4. Discussion 34 Reference 50 Chapter II Physico-immunological Response of the Bay Scallop (Argopecten irradians) Exposed to the Algicide Palmitoleic Acid 56 Abstract 56 2.1. Introduction 58 2.2. Materials and methods 61 2.3. Results 65 2.4. Discussion 69 References 84 Chapter III Immune Toxicological Effect of Marine Toxin Okadaic Acid in Bay Scallop (Argopecten irradians) 91 Abstract 91 3.1. Introduction 93 3.2. Materials and Methods 96 3.3. Results 101 3.4. Discussion 104 References 119 Chapter IV Antioxidant and Non-specific Immune Responses of Bay Scallop (Argopecten irradians) Exposed to Marine Toxin Okadaic Acid 126 Abstract 126 4.1. Introduction 128 4.2. Materials and methods 131 4.3. Results 135 4.4. Discussion 138 References 151 General conclusion 156 Abstract in Korean 158 List of published articles 162 List of conference attendance 167 Acknowledgements 172 | - |
dc.format | application/pdf | - |
dc.format.extent | 1715876 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Mollusks | - |
dc.subject | Immunological Response | - |
dc.subject | Bay Scallop | - |
dc.subject | Algicide | - |
dc.subject | Algal Toxin | - |
dc.subject | Palmitoleic Acid | - |
dc.subject | Okadaic Acid | - |
dc.subject.ddc | 636.089 | - |
dc.title | Physico-immunological Characterizations of Exogenous Substances (Palmitoleic Acid and Okadaic Acid) in Bivalves | - |
dc.title.alternative | 이매패류에 있어 외인성 화합물(Palmitoleic Acid와 Okadaic Acid)에 의한 생리면역학적 특성 규명 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Chi Cheng | - |
dc.description.degree | Doctor | - |
dc.contributor.affiliation | 수의과대학 수의학과 | - |
dc.date.awarded | 2017-08 | - |
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