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Functional analysis of Transcription Factors containing CCAAT-DNA Binding domain in Fusarium graminearum : 식물병원성곰팡이 Fusarium graminearum의 CCAAT DNA 결합부위를 가진 전사조절인자 FCT1과 FCT3의 기능과 상호작용분석
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 이인원 | - |
dc.contributor.author | 남혜진 | - |
dc.date.accessioned | 2017-10-31T07:51:16Z | - |
dc.date.available | 2017-10-31T07:51:16Z | - |
dc.date.issued | 2017-08 | - |
dc.identifier.other | 000000145676 | - |
dc.identifier.uri | https://hdl.handle.net/10371/137575 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 농업생명과학대학 농생명공학부, 2017. 8. 이인원. | - |
dc.description.abstract | The homothallic ascomycete fungus Fusarium graminearum is an important plant pathogen of major cereal crops. The CCAAT sequence is present in roughly 30% of eukaryotic promoters. In filamentous fungi, the CCAAT sequence has been known to modulate the expression of several critical genes involved in various developmental stages. Eight transcription factors (TFs) containing CCAAT-DNA binding domain (FCT1~8) are in F. graminearum. Among them, we chose 2 TF
mutants, ∆fct1 and ∆fct3, that have similar pleiotropic defects from a previously generated TF mutant library. Both deletion mutants have similar defects in mycelia growth, sexual reproduction, and virulence. Double deletion of FCT1 and FCT3 resulted in indistinguishable phenotypes compared to each single deletion mutant. Moreover, Fct1 and Fct3 physically interacted with each other and localized to nuclei, suggesting that Fct1 and Fct3 form protein complex for transcriptional regulation. This is the first study dealing with TFs containing CCAAT DNA-binding domain in plant pathogenic fungi and present weighty perception to understand molecular mechanisms underlying functions of CCAAT-binding factors in F. graminearum. | - |
dc.description.tableofcontents | INTRODUCTION 1
MATERIALS AND METHODS 3 1. Fungal strains and media 3 2. Genetic manipulations, primers, and sequencing 5 3. Targeted deletion and complementation 9 4. Quantitative real time (qRT)-PCR 12 5. Cellular localization of Fct1 and Fct3 13 6. Sexual crosses 14 7. Virulence test and trichothecenes analysis 14 8. Yeast-two-hybrid assay 15 RESULTS 17 1. Identification of CCAAT-DNA binding transcription factors FCT1 and FCT3 17 2. Vegetative growth and perithecial development 19 3. Cellular localization of Fct1 and Fct3 19 4. Expression of FCT1 and FCT3 20 5. FCT1 and FCT3 are involved in pathogenicity during infection of wheat 25 6. FCT1 and FCT3 have an effect on the biosynthesis of trichothecenes 28 7. Fct1 interacts with Fct3 30 DISCUSSION 32 REFERENCES 36 | - |
dc.format | application/pdf | - |
dc.format.extent | 4161132 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | CCAAT | - |
dc.subject | DNA binding domain | - |
dc.subject | Fusarium graminearum | - |
dc.subject | transcription factor | - |
dc.subject.ddc | 630 | - |
dc.title | Functional analysis of Transcription Factors containing CCAAT-DNA Binding domain in Fusarium graminearum | - |
dc.title.alternative | 식물병원성곰팡이 Fusarium graminearum의 CCAAT DNA 결합부위를 가진 전사조절인자 FCT1과 FCT3의 기능과 상호작용분석 | - |
dc.type | Thesis | - |
dc.description.degree | Master | - |
dc.contributor.affiliation | 농업생명과학대학 농생명공학부 | - |
dc.date.awarded | 2017-08 | - |
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