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Noninvasive method of immortalized neural stem-like cell transplantation in an experimental model of Huntington's disease

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Authors
Lee, Soon-Tae; Park, Jung-Eun; Lee, Kyungmi; Kang, Lami; Chu, Kon; Kim, Seung U; Kim, Manho; Roh, Jae-Kyu
Issue Date
2005-11-01
Publisher
Elsevier
Citation
J Neurosci Methods. 2006 Apr 15;152(1-2):250-4. Epub 2005 Oct 28.
Keywords
AnimalsCell Line, Transformed/*transplantationCell MovementHumansHuntington Disease/chemically induced/*therapyImmunohistochemistryImmunosuppressionInjections, IntraventricularLac Operon/geneticsMaleNeurons/*transplantationQuinolinic Acid/toxicityRatsRats, Sprague-DawleyStem Cell Transplantation/*methodsStereotaxic Techniques
Abstract
A loss of neostriatal neurons is a characteristic of Huntington's disease (HD), and neural tissue transplantation has been performed directly into the striatum. Since the neural stem cells have ability to migrate into the lesion site, we administered immortalized neural stem-like cells (NSC) into the ventricle or via a tail vein following unilateral intrastriatal quinolinic acid lesioning in Sprague-Dawley rats. To identify transplanted NSC, cells were encoded with lac Z and beta-galactosidase histochemistry was performed. lac Z+ cells were detected in the lesioned striatum but tissue damage or tumor formation was not observed. This study shows that NSC migrate into the striatum, either from ventricle or from the systemic circulation, providing less invasive routes for stem cell application in HD.
ISSN
0165-0270 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16257450

http://hdl.handle.net/10371/13762
DOI
https://doi.org/10.1016/j.jneumeth.2005.09.013
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Neurology (신경과학교실)Journal Papers (저널논문_신경과학교실)
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