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Comparative genomic analysis and characteristics of NCCP15740, the major type of enterotoxigenic Escherichia coli in Korea

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Authors
Kwon, Taesoo; Chung, Si-yun; Jung, Young-Hee; Jung, Su-Jin; Roh, Sang-Gyun; Park, Je-Seop; Kim, Cheorl-Ho; Kim, Won; Bak, Young-Seok; Cho, Seung-Hak
Issue Date
2017-09-20
Publisher
BioMed Central
Citation
Gut Pathogens, 9(1):55
Keywords
Enterotoxigenic Escherichia coli O6Whole-genome sequencingColonization factor genesEnterotoxinMultilocus sequence typingVirulence
Description
BSR: BLAST score ratio; CDS: coding DNA sequences; CF: colonization factor; ETEC: enterotoxigenic Escherichia coli; LT: heat-labile toxin; STh: heat-stable toxin; MLST: multilocus sequence typing; NCCP: national culture collection for pathogens; RAST: rapid annotation using subsystem technology; str.: train.
Abstract
Abstract

Background
Enterotoxigenic Escherichia coli (ETEC) cause infectious diarrhea and diarrheal death. However, the genetic properties of pathogenic strains vary spatially and temporally, making prevention and treatment difficult. In this study, the genomic features of the major type of ETEC in Korea from 2003 to 2011 were examined by whole-genome sequencing of strain NCCP15740, and a comparative genomic analysis was performed with O6 reference strains.

Results
The assembled genome size of NCCP15740 was 4,795,873 bp with 50.54% G+C content. Using rapid annotation using subsystem technology analysis, we predicted 4492 ORFs and 17 RNA genes. NCCP15740 was investigated for enterotoxin genes, colonization factor (CF) genes, serotype, multilocus sequence typing (MLST) profiles, and classical and nonclassical virulence factors. NCCP15740 belonged to the O6:H16 serotype and possessed enterotoxin genes encoding heat-stable toxin (STh) and heat-labile toxin (LT); 87.5% of the O6 serotype strains possessed both toxin types. NCCP15740 carried the colonization factors CS2 and CS3, whereas most O6 strains carried CS2-CS3-CS21 (79.2%). NCCP15740 harbored fewer virulence factors (59.4%) than the average observed in other O6 strains (62.0%). Interestingly, NCCP15740 did not harbor any nonclassical virulence genes.

Conclusions
The major type of ETEC in Korea had the same MLST sequence type as that of isolates from the USA obtained in 2011 and 2014, but had different colonization factor types and virulence profiles. These results provide important information for the development of an ETEC vaccine candidate.
ISSN
1757-4749
Language
English
URI
http://hdl.handle.net/10371/138303
DOI
https://doi.org/10.1186/s13099-017-0204-y
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College of Natural Sciences (자연과학대학)Program in Bioinformatics (협동과정-생물정보학전공)Journal Papers (저널논문_협동과정-생물정보학전공)
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